Improved, low-cost selective culture medium for Actinobacillus actinomycetemcomitans
Improved, low-cost selective culture medium for Actinobacillus actinomycetemcomitans
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DOI：
10.1128/jcm.39.2.509-513.2001
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发表时间：
2001-02-01
影响因子：
9.4
通讯作者：
Frias, J
中科院分区：
文献类型：
--
作者：
Alsina, M;Olle, E;Frias, J
伴放线放线杆菌被认为是主要的口腔假定病原体之一，特别是在幼年牙周炎的情况下，这种微生物需要营养复杂的培养基才能生长，因此其初级分离的培养基通常包括血琼脂或血清作为基础。在这项研究中，我们提出了一种新的培养基，Dentaid-1，它提高了牙周样品中放线放线杆菌的检测。在其成分中，省略了血液和血清，从而降低了其成本，并使其成为对其他具有高营养要求的微生物生长的限制性更强的培养基。细菌纯培养物在 Dentaid-1 上的生长产量与非选择性血琼脂上的生长产量相当。此外，还对 77 名成人牙周炎受试者的龈下样本进行了临床疗效评估。 Dentaid-1 在 24 名受试者中检测到 A. actinomycetemcomitans，而之前描述的胰蛋白酶大豆-血清-杆菌肽-万古霉素琼脂仅在 19 名受试者 (79.1%) 中检测到该微生物，Dentaid-1 是一种低成本、非抑制性配方，用于改进对这种重要口腔假定病原体龈下感染的患者的诊断和监测。
Actinobacillus actinomycetemcomitans is considered to be one of the major oral putative pathogens, especially in cases of juvenile periodontitis, This microorganism requires nutritionally complex media for growth, and therefore the media for its primary isolation usually include blood agar or serum in their base, In this study we present a new medium, Dentaid-1, which improves the detection of A. actinomycetemcomitans in periodontal samples. In its composition, blood and serum have been omitted, hence reducing its cost and making it a more restrictive medium against the growth of other microorganisms with high nutritional requirements. The growth yields of pure cultures of the bacteria on Dentaid-1 were comparable to those on nonselective blood agar. Moreover, clinical efficacy was evaluated in subgingival samples from 77 subjects with adult periodontitis. Dentaid-1 detected A. actinomycetemcomitans in 24 subjects, while a previously described tryptic soy-serum-bacitracin-vancomycin agar detected the microorganism in only 19 subjects (79.1%), Dentaid-1 is a low-cost, noninhibitory formula for the improved diagnosis and monitoring of patients subgingivally infected by this important oral putative pathogen.