Effects of Salmonella enterica serovar Enteritidis on cellular recruitment and cytokine gene expression in caecum of vaccinated chickens
Effects of Salmonella enterica serovar Enteritidis on cellular recruitment and cytokine gene expression in caecum of vaccinated chickens
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DOI：
10.1016/j.vaccine.2008.07.088
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发表时间：
2008-10-03
期刊：
影响因子：
5.5
通讯作者：
Berndt, Angela
中科院分区：
文献类型：
--
作者：
Carvajal, Barbara Gonzalez;Methner, Ulrich;Berndt, Angela
虽然家禽疫苗接种是限制沙门氏菌引起的人类食源性胃肠炎的合适方法，但对鸟类沙门氏菌感染产生更持久保护作用的免疫机制尚不完全清楚。为了揭示独特的保护相关免疫参数，研究人员在雏鸡出生后第56天接种了商业肠炎沙门氏菌活疫苗，并对野生型肠炎沙门氏菌147N进行了攻击。采用免疫组织化学和实时定量RT-PCR技术分析盲肠标本的免疫细胞组成和细胞因子基因表达谱。目前的数据表明，疫苗引发的对野生型沙门氏菌感染的免疫保护与肠道黏膜和肝脏中的细菌计数有关，而不是与肠道内的定植有关。攻毒后，未接种疫苗的鸡盲肠壁和肝脏中沙门氏菌野生型生物的数量高于接种疫苗的鸡，这与IL-8、LITAF、iNOS、IL-12和ifn - γ的基因表达率显著相关。相比之下，免疫后的鸡在盲肠感染肠炎沙门氏菌147N后，CD8(+) t细胞和IgA的含量高于未免疫的鸡。结果表明，与未接种疫苗的鸡相比，先前接种疫苗的鸡在肠炎沙门氏菌野生型攻击下具有独特的免疫反应模式。(C) 2008年Elsevier Ltd.出版
Although vaccination of poultry is a suitable method to limit human food borne gastroenteritis caused by Salmonella (S.), the immune mechanisms responsible for a longer lasting protection against Salmonella infection in birds are not completely understood. To reveal unique protection-related immune parameters, day-old chicks were vaccinated with a commercial live S. Enteritidis vaccine and challenged with wild-type S. Enteritidis 147N at day 56 of life. The bacterial cell count was determined in gut and liver, while the immune cell composition and cytokine gene expression patterns were analysed by immunohistochemistry and quantitative real-time RT-PCR in caecum samples.The presented data suggest that the vaccine-elicited immune protection against the Salmonella wildtype infection was rather related to the bacterial count in gut mucosa and liver than to the colonisation in gut lumen. The higher number of Salmonella wild-type organisms found in caecal wall and liver of the non-immunised compared to immunised birds after challenge correlated with a more pronounced gene expression rate for IL-8, LITAF, iNOS, IL-12 and IFN-gamma. In contrast, immunised birds exhibited higher amounts of CD8(+) Tcells as well as IgA than the non-immunised chickens after S. Enteritidis 147N infection in caecum.The results demonstrated a distinctive immune reaction pattern of previously vaccinated compared to non-vaccinated chickens upon S. Enteritidis wild-type challenge. (C) 2008 Published by Elsevier Ltd.