Erythrocyte membrane-camouflaged polymeric nanoparticles as a biomimetic delivery platform
Erythrocyte membrane-camouflaged polymeric nanoparticles as a biomimetic delivery platform
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DOI：
10.1073/pnas.1106634108
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发表时间：
2011-07-05
影响因子：
11.1
通讯作者：
Zhang, Liangfang
中科院分区：
文献类型：
--
作者：
Hu, Che-Ming J.;Zhang, Li;Zhang, Liangfang
延长纳米颗粒在体内停留时间的努力已经激发了颗粒表面修饰以绕过巨噬细胞摄取和全身清除的许多策略。在这里，我们报告了一个自上而下的仿生方法在颗粒功能化涂层可生物降解的聚合物纳米粒子与天然红细胞膜，包括膜脂质和相关的膜蛋白的长循环货物交付。红细胞膜包被的纳米颗粒的结构，大小和表面zeta电位，和蛋白质含量分别采用透射电子显微镜，动态光散射，凝胶电泳进行验证。与涂有最先进的合成隐形材料的对照颗粒相比，用装载有荧光团的纳米颗粒注射小鼠显示出红细胞模拟纳米颗粒的上级循环半衰期。生物分布研究显示，在颗粒注射后72小时，血液中有显著的颗粒保留。天然细胞膜、它们的相关蛋白质和相应的功能性转移到合成颗粒的表面代表了纳米颗粒功能化的独特方法。
Efforts to extend nanoparticle residence time in vivo have inspired many strategies in particle surface modifications to bypass macrophage uptake and systemic clearance. Here we report a top-down biomimetic approach in particle functionalization by coating biodegradable polymeric nanoparticles with natural erythrocyte membranes, including both membrane lipids and associated membrane proteins for long-circulating cargo delivery. The structure, size and surface zeta potential, and protein contents of the erythrocyte membrane-coated nanoparticles were verified using transmission electron microscopy, dynamic light scattering, and gel electrophoresis, respectively. Mice injections with fluorophore-loaded nanoparticles revealed superior circulation half-life by the erythrocyte-mimicking nanoparticles as compared to control particles coated with the state-of-the-art synthetic stealth materials. Biodistribution study revealed significant particle retention in the blood 72 h following the particle injection. The translocation of natural cellular membranes, their associated proteins, and the corresponding functionalities to the surface of synthetic particles represents a unique approach in nanoparticle functionalization.