Software for precise tracking of cell proliferation
Software for precise tracking of cell proliferation
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DOI：
10.1016/j.bbrc.2011.12.100
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发表时间：
2012-01-20
影响因子：
3.1
通讯作者：
Miyawaki, Atsushi
中科院分区：
文献类型：
--
作者：
Kurokawa, Hiroshi;Noda, Hisayori;Miyawaki, Atsushi
我们开发了一个多目标细胞跟踪程序TADOR，我们将其应用于一系列荧光图像。TADOR基于活动轮廓模型，该模型被修改以避免局部最优解的问题，从而抵抗信号波动和形态变化。由于采用了反向跟踪和增加了用户交互校正功能，TADOR以离线和半自动模式使用，但能够精确跟踪细胞分裂。通过将TADOR应用于其细胞核已被荧光标记的培养细胞的分析，我们在长时间内跟踪盖玻片上的细胞分裂和细胞周期进展。(C)2011 Elsevier Inc. All rights reserved.
We have developed a multi-target cell tracking program TADOR, which we applied to a series of fluorescence images. TADOR is based on an active contour model that is modified in order to be free of the problem of locally optimal solutions, and thus is resistant to signal fluctuation and morphological changes. Due to adoption of backward tracing and addition of user-interactive correction functions, TADOR is used in an off-line and semi-automated mode, but enables precise tracking of cell division. By applying TADOR to the analysis of cultured cells whose nuclei had been fluorescently labeled, we tracked cell division and cell-cycle progression on coverslips over an extended period of time. (C) 2011 Elsevier Inc. All rights reserved.