Fibrous nanostructures from the self-assembly of designed repeat protein modules.
Fibrous nanostructures from the self-assembly of designed repeat protein modules.
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DOI：
10.1002/anie.201203795
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发表时间：
2012-12
影响因子：
--
通讯作者：
J. Phillips;C. Millership;E. Main
中科院分区：
文献类型：
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作者：
J. Phillips;C. Millership;E. Main
通过控制单体重复蛋白末端界面的化学成分和溶剂暴露，可以得到单链超螺旋微丝。该组装是在水溶液中，在中性pH值下，在室温下完成的。该构建块是一个重组工程设计的四肽重复蛋白。定向的头到尾的自组装是由遗传编码的正交天然化学连接驱动的。
Single-protein-chain superhelical filaments are obtained from monomeric repeat proteins by controlling the chemistry and solvent exposure at their terminal interfaces. The assembly was achieved in aqueous solution, at neutral pH value, and at room temperature. The building block was a recombinantly engineered designed tetratricopeptide repeat protein. Directed head-to-tail self-assembly was driven by genetically encoded orthogonal native chemical ligation.