ClassII peroxidase-encoding genes are present in a phylogenetically wide range of ectomycorrhizal fungi
ClassII peroxidase-encoding genes are present in a phylogenetically wide range of ectomycorrhizal fungi
复制标题
DOI：
10.1038/ismej.2009.77
复制
发表时间：
2009-12-01
期刊：
影响因子：
11
通讯作者：
Lindahl, Bjorn D.
中科院分区：
文献类型：
--
作者：
Bodeker, Inga T. M.;Nygren, Cajsa M. R.;Lindahl, Bjorn D.
真菌过氧化物酶（i类）在北方森林木材、凋落物和腐殖质中降解顽固性多酚类化合物具有关键作用。迄今为止，它们的发生和活动主要是在少数白腐木材分解者中进行的研究。然而，过氧化物酶活性通常在北方森林腐殖质和矿质土壤中测量，其中外生菌根真菌占主导地位。在这里，我们使用退化PCR引物来研究过氧化物酶编码基因是否存在于广泛的系统发育范围的外生菌根类群的基因组中。PCR产物的克隆和测序结果表明，来自不同属的外生菌根真菌具有过氧化物酶基因。这些新序列代表了4个主要的同担子菌系，但大多数来自Cortinarius、Russula和Lactarius。这些属在生态学上是重要的，但主要由不可培养的物种组成，从这些物种中获得的生态生理学信息很少。扩增的序列包含保守的折叠和底物氧化活性位点。在一些海螺属中，有证据表明在该属的进化过程中存在基因复制。分类过氧化物酶似乎是一种古老的和共同的特征，大多数同质孢子菌，包括外生菌根真菌。胞外过氧化物酶的产生可能为外生菌根真菌提供了获取氮的途径，这些氮被隔离在复杂的多酚源中。ISME学报（2009）3,1387-1395；doi: 10.1038 / ismej.2009.77;2009年7月2日在线发布
Fungal peroxidases (ClassII) have a key role in degrading recalcitrant polyphenolic compounds in boreal forest wood, litter and humus. To date, their occurrence and activity have mainly been studied in a small number of white-rot wood decomposers. However, peroxidase activity is commonly measured in boreal forest humus and mineral soils, in which ectomycorrhizal fungi predominate. Here, we used degenerate PCR primers to investigate whether peroxidase-encoding genes are present in the genomes of a wide phylogenetic range of ectomycorrhizal taxa. Cloning and sequencing of PCR products showed that ectomycorrhizal fungi from several different genera possess peroxidase genes. The new sequences represent four major homobasidiomycete lineages, but the majority is derived from Cortinarius, Russula and Lactarius. These genera are ecologically important, but consist mainly of non-culturable species from which little ecophysiological information is available. The amplified sequences contain conserved active sites, both for folding and substrate oxidation. In some Cortinarius spp., there is evidence for gene duplications during the evolution of the genus. ClassII peroxidases seem to be an ancient and a common feature of most homobasidiomycetes, including ectomycorrhizal fungi. Production of extracellular peroxidases may provide ectomycorrhizal fungi with access to nitrogen sequestered in complex polyphenolic sources. The ISME Journal (2009) 3, 1387-1395; doi: 10.1038/ismej.2009.77; published online 2 July 2009