L-selectin and E-selectin expressed on monocytes mediating Ehrlichia chaffeensis attachment onto host cells.
L-selectin and E-selectin expressed on monocytes mediating Ehrlichia chaffeensis attachment onto host cells.
复制标题
L-选择素和E-选择素在单核细胞上表达，介导恰菲埃里希体附着到宿主细胞上。
DOI：
10.1016/s0378-1097(03)00696-7
复制
发表时间：
2003
影响因子：
2.1
通讯作者：
Yu,Xue-jie
中科院分区：
文献类型：
--
作者：
Zhang,Jian-zhi;McBride,JereW;Yu,Xue-jie
查菲埃立克体是引起人类单核细胞埃立克体病的病原体，是一种专性细胞内细菌，具有嗜单核细胞宿主细胞性。埃立克体必须进入宿主细胞，然后建立感染。E. chaffeensisfor单核细胞表明，细胞含有一些特定的表面成分，介导E。chaffeensis附着和进入宿主细胞。在这项研究中，宿主细胞表面的成分，在埃里希体附着的作用进行了鉴定，使用人单核细胞/巨噬细胞系，THP-1.E。用真实的时间聚合酶链反应测定，E-选择素和L-选择素抗体可部分阻断chaffeensis-THP-1细胞的粘附，而P-选择素、整合素αm、整合素αx或正常小鼠IgG抗体则不能阻断chaffeensis-THP-1细胞的粘附。相反，在不表现出E-选择素和L-选择素的表面表达的HeLa细胞中，针对这些细胞表面蛋白的抗体不抑制E。chaffeensisattachment.这些发现表明，E-选择素和L-选择素是细胞表面蛋白，可能作为共受体，并有助于E。chaffeensisattachment和entry进入THP-1。
Ehrlichia chaffeensis, the agent of human monocytic ehrlichiosis, is an obligatory intracellular bacterium that exhibits monocytic host cell tropism. Ehrlichiae must enter the host cell, and then establish infection. The tropism ofE. chaffeensisfor monocytes suggests that the cell contains some specific surface components that mediateE. chaffeensisattachment and entry into host cells. In this study, host cell surface components that play a role in ehrlichial attachment were identified using a human monocyte/macrophage cell line, THP-1.E. chaffeensisattachment to THP-1 cells was partially blocked in the presence of antibodies to E-selectin and L-selectin, but not by antibodies to P-selectin, integrin αm, integrin αx, or normal mouse IgG as determined by real time polymerase chain reaction. Conversely, in HeLa cells that do not exhibit surface expression of E-selectin and L-selectin, antibodies to these cell surface proteins did not inhibitE. chaffeensisattachment. These findings indicate that E-selectin and L-selectin are cell surface proteins that might act as co-receptors and contribute toE. chaffeensisattachment and entry into THP-1.