Developing a new model system for potato genetics by androgenesis
Developing a new model system for potato genetics by androgenesis
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通过雄激素发生开发马铃薯遗传学新模型系统
DOI：
10.1111/jipb.13018
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发表时间：
2021-03-04
影响因子：
11.4
通讯作者：
Qi, Jianjian
中科院分区：
文献类型：
--
作者：
Du, Miru;Wang, Ting;Qi, Jianjian
高杂合性和四体遗传使马铃薯等无性繁殖多倍体的研究复杂化。反向遗传学方法，特别是突变体库的构建，可以是一个理想的选择，如果一个合适的诱变基因型是可用的。在这里，我们的目的是产生一个模式系统，马铃薯研究使用花药培养的茄疣，自交亲和二倍体马铃薯具有很强的晚疫病抗性。获得的23个再生体中有6个（SVA 4、SVA 7、SVA 22、SVA 23、SVA 32和SVA 33）是二倍体，它们的纯合性估计>99.99%，具有22个多态性InDel标记。两个品系-SVA 4和SVA 32-具有降低的株高（植物高度1，000粒种子/植物）和良好的块茎形成（>30个块茎/植物）。我们使用全基因组重测序进一步证实了SVA 4和SVA 32的完全纯合性。这两个再生体具有模式植物的所有特征：二倍体、100%纯合性、自交亲和性和可转基因性。因此，我们已经成功地产生了两个品系，SVA 4和SVA 32，它们可以潜在地用于诱变和作为模式植物来恢复当前进行马铃薯研究的方法。
High heterozygosity and tetrasomic inheritance complicate studies of asexually propagated polyploids, such as potato. Reverse genetics approaches, especially mutant library construction, can be an ideal choice if a proper mutagenesis genotype is available. Here, we aimed to generate a model system for potato research using anther cultures of Solanum verrucosum, a self-compatible diploid potato with strong late blight resistance. Six of the 23 regenerants obtained (SVA4, SVA7, SVA22, SVA23, SVA32, and SVA33) were diploids, and their homozygosity was estimated to be >99.99% with 22 polymorphic InDel makers. Two lines-SVA4 and SVA32-had reduced stature (plant height 1,000 seeds/plant), and good tuber set (>30 tubers/plant). We further confirmed the full homozygosity of SVA4 and SVA32 using whole-genome resequencing. These two regenerants possess all the characteristics of a model plant: diploidy, 100% homozygosity, self-compatibility, and amenability to transgenesis. Thus, we have successfully generated two lines, SVA4 and SVA32, which can potentially be used for mutagenesis and as model plants to rejuvenate current methods of conducting potato research.