Nucleotide sequence of the tripartite Fragaria chiloensis cryptic virus and presence of the virus in the Americas
Nucleotide sequence of the tripartite Fragaria chiloensis cryptic virus and presence of the virus in the Americas
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DOI：
10.1007/s11262-007-0186-9
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发表时间：
2008-02-01
期刊：
影响因子：
1.6
通讯作者：
Martin, Robert R.
中科院分区：
文献类型：
--
作者：
Tzanetakis, Ioannis E.;Price, Ruth;Martin, Robert R.
用于表征智利草莓潜伏病毒（FCILV）的植物含有双链RNA（dsRNA）条带，其大小与已知草莓病毒的基因组或亚基因组RNA不对应。利用鸟枪法克隆获得了智利草莓隐病毒（Fragaria chiloensis cryptic virus，FCCV）的全序列。本通讯提供了FCCV的完整基因组序列。FCCV的基因组由三个单顺反子RNA组成，这对于通常是二分的隐蔽病毒来说是不寻常的特征。最大的分子编码RNA依赖的RNA聚合酶，而其他两个编码密切相关的蛋白质，预计是病毒的外壳蛋白。系统发育分析表明，FCCV是最密切相关的真菌感染Partitivirus属的成员，而不是植物感染Alphacryptovirus的成员，提供了一个可能的洞察到的进化的隐蔽病毒。对来自北美和南美的300多株草莓植物进行了FCCV检测，仅在南美的植物中检测到该病毒。
The plant used in the characterization of Fragaria chiloensis latent virus (FClLV) contained double-stranded RNA (dsRNA) bands that did not correspond in size to the genomic or subgenomic RNAs of known strawberry viruses. Using shotgun cloning, sequences of a cryptic virus, named Fragaria chiloensis cryptic virus (FCCV), were obtained. This communication presents the complete genome sequence of FCCV. The genome of FCCV consists of three monocistronic RNAs, an unusual feature for cryptic viruses that are normally bipartite. The largest molecule encodes an RNA-dependent RNA polymerase, while the other two encode closely related proteins predicted to be the coat proteins of the virus. Phylogenetic analysis showed that FCCV is related most closely to members of the fungi-infecting Partitivirus genus rather than members of the plant-infecting Alphacryptovirus, providing a possible insight into the evolution of cryptic viruses. More than 300 strawberry plants from North and South America were tested for FCCV, and the virus was only detected in plants from South America.