Comparison Evans Blue injection routes: Intravenous versus intraperitoneal, for measurement of blood-brain barrier in a mice hemorrhage model.
Comparison Evans Blue injection routes: Intravenous versus intraperitoneal, for measurement of blood-brain barrier in a mice hemorrhage model.
复制标题
DOI：
10.1016/j.jneumeth.2010.12.013
复制
发表时间：
2011-02-15
影响因子：
3
通讯作者：
Tang, Jiping
中科院分区：
文献类型：
--
作者：
Manaenko, Anatol;Chen, Hank;Kammer, Jerome;Zhang, John H.;Tang, Jiping
脑出血是中风最具破坏性的亚型之一，使幸存者患有严重的神经功能缺损。出血后血脑屏障（BBB）的破坏导致血管源性脑水肿的发展，这是继脑出血（ICH）等事件之后最危及生命的事件。伊文思蓝测定是一种流行的 BBB 破坏定量方法。尽管这种方法很常用，但文献中有几种测定方案，它们的给药途径以及染色剂的循环时间各不相同。在这项研究中，我们比较了腹腔注射与静脉注射后循环时间 0.5、3 和 24 小时脑组织中累积的染色量。使用58只CD-1小鼠。将动物分为 ICH (N=42)、假手术组 (N=6) 和初始组 (N=10)。 ICH 动物的右侧基底神经节接受 VII 型胶原酶立体定向注射。假手术动物仅接受针刺创伤。注射胶原酶或针外伤后 24 小时注射伊文思蓝染色剂。 The consistency of ICH produced was characterized by estimation of hematoma volume via hemoglobin assay and neurological evaluation.不同实验组之间产生的血肿和神经功能缺陷具有很好的可比性。不同给药途径的伊文思蓝测定结果没有统计学上的显着差异。胶原酶注射产生ICH后，伊文思蓝染色剂在小鼠脑中积累的量与染色剂施用途径无关。
Intracerebral hemorrhage is one of the most devastating subtypes of stroke, leaving survivors with severe neurological deficits. Disruption of the blood brain barrier (BBB) following hemorrhage results in development of vasogenic brain edema, a most life-threatening event after such events as intracerebral hemorrhage (ICH). The Evans Blue assay is a popular method for the quantification of BBB disruption. Although this method is in common use, there are several protocols of the assay in the literature which vary in the route of administration, as well as the circulation time of the stain. In this study, we compared the amounts of accumulated stain in brain tissue following intraperitoneal versus intravenous injection at 0.5, 3 and 24 hours of circulation time. 58 CD-1 mice were used. Animals were divided into ICH (N=42), sham groups (N= 6) and naïve (N=10). ICH animals received stereotactic injection of collagenase type VII into the right basal ganglia. Sham animals received only needle trauma. Evans Blue stain was injected 24 hours after collagenase injection or needle trauma. The consistency of ICH produced was characterized by estimation of hematoma volume via hemoglobin assay and neurological evaluation. The produced hematoma and neurological deficits were well comparable between different experimental groups. There was no statistically significant difference in the results of the Evans Blue assay with regard to administration route. The amount of Evans Blue stain accumulated in the brains of mice after ICH produced by collagenase injection was independent of the stain administration route.
登录
查看更多内容
Aquaporin-4对脑出血后水肿形成的影响
影响因子：
5.3
作者：
Tang, Yuping;Wu, Pin;Dong, Qiang
通讯作者：
Dong, Qiang
影响因子：
6.9
作者：
Wu B;Ma Q;Khatibi N;Chen W;Sozen T;Cheng O;Tang J
通讯作者：
Tang J
影响因子：
6.3
作者：
Foerch, Christian;Arai, Ken;Lo, Eng H.
通讯作者：
Lo, Eng H.
影响因子：
8.3
作者：
Belayev, L;Saul, I;Ginsberg, MD
通讯作者：
Ginsberg, MD
影响因子：
4.1
作者：
Kim, Dong Wook;Im, So-Hyang;Jeong, Sang-Wuk
通讯作者：
Jeong, Sang-Wuk