Molecular characterization of SLG and S-related genes in a self-compatible Brassica campestris L. var. yellow sarson
Molecular characterization of SLG and S-related genes in a self-compatible Brassica campestris L. var. yellow sarson
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自交亲和甘蓝中 SLG 和 S 相关基因的分子特征
DOI：
10.1007/s004970050107
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发表时间：
1997
影响因子：
--
通讯作者：
K. Hinata
中科院分区：
文献类型：
--
作者：
M. Watanabe;T. Ono;K. Hatakeyama;S. Takayama;A. Isogai;K. Hinata
摘要 来自Brassica Campestris var.的自交亲和菌株C636的柱头cDNA文库。 Yellow sarson，我们分离了三个 cDNA 克隆，它们与编码 SLG 或 SLR 的 cDNA 具有高度的序列相似性。这三个 cDNA 克隆分别命名为 SLG (C636)、SLR1 (C636) 和 SLR2 (C636)。对 C636 和自交不亲和 S8 纯合子之间的杂交体的分离 F2 后代进行限制性片段长度多态性 (RFLP) 连锁分析，结果显示 SLG (C636) 与 S 基因座连锁，而 SLR1 (C636) 和 SLR2 (C636) 则不然。后两个基因彼此不相关。在 C636 的柱头中检测到 SLG (C636)、SLR1 (C636) 和 SLR2 (C636) 转录本，但在花药中未检测到。然而，SLG (C636)转录物的稳态水平显着低于自交不亲和的S9纯合子中SLG转录物的稳态水平。在 C636 的柱头组织中未检测到 SRK 转录物，而在自交不亲和的 S9 纯合子中检测到 SRK 转录物预期大小的 RNA 带。通过免疫印迹分析，在 C636 和其他三种自交亲和黄沙森菌株中检测到 SLG 蛋白；然而，在自交不亲和菌株中，其含量低于 SLG 的含量。我们得出结论，C636 黄沙森自交性破坏的原因之一是 SLG 基因的下调和/或 SRK 基因表达的失败。
Abstract From a stigma cDNA library of a self-compatible strain, designated C636, of Brassica campestris var. yellow sarson, we isolated three cDNA clones that showed a high degree of sequence similarity with cDNAs encoding either SLG or SLR’s. These three cDNA clones were designated SLG (C636), SLR1 (C636), and SLR2 (C636), respectively. Restriction fragment length polymorphism (RFLP) linkage analyses of a segregating F2 progeny of a hybrid between C636 and the self-incompatible S8-homozygote revealed that SLG (C636) was linked to the S locus, whereas SLR1 (C636) and SLR2 (C636) were not. The latter two genes were not linked to each other. The transcripts of SLG (C636), SLR1 (C636), and SLR2 (C636) were detected in stigmas, but not in anthers, of C636. However, the steady-state level of the SLG (C636) transcript was significantly lower than that of the SLG transcript in the self-incompatible S9-homozygote. No SRK transcripts were detected in the stigma tissue of C636, whereas an RNA band of the expected size of the SRK transcript was detected in the self-incompatible S9-homozygote. The SLG protein was detected in C636 and in three other self-compatible yellow sarson strains by immunoblot analysis; however, the amounts were lower than those of SLGs in self-incompatible strains. We conclude that one reason for the breakdown of self-compatibility in C636 yellow sarson is the down-regulation of the SLG gene and/or failure of the expression of the SRK gene.