POPULATION-DYNAMICS OF PLASMODIUM-FALCIPARUM SPOROGONY IN LABORATORY-INFECTED ANOPHELES-GAMBIAE
POPULATION-DYNAMICS OF PLASMODIUM-FALCIPARUM SPOROGONY IN LABORATORY-INFECTED ANOPHELES-GAMBIAE
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DOI：
10.2307/3283550
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发表时间：
1992-08-01
影响因子：
1.3
通讯作者：
BEIER, JC
中科院分区：
文献类型：
--
作者：
VAUGHAN, JA;NODEN, BH;BEIER, JC
本文研究了恶性疟原虫在冈比亚按蚊体内孢子生殖发育过程中的种群动态。确定了每个生命阶段的绝对密度估计值，并为38个实验感染中的每一个构建了生命表。巨配子体和动合子死亡率对总体死亡率的贡献相等。平均而言，在从巨配子体到动合子阶段的过渡中，寄生虫数量减少40倍，在从动合子到卵囊阶段的过渡中减少69倍，并且从巨配子体到卵囊阶段的寄生虫数量总净减少2，754倍（即，乘法）。由于不同配子体培养物之间的生育力固有变异性，巨配子体和动合子密度之间没有关系。动合子和卵囊密度之间存在曲线关系（r2 = 0.66）。在约30个动合子/蚊子的阈值以上，每个动合子的卵囊产量随着动合子密度的增加而变得越来越大。平均每个卵囊产生663个唾液腺子孢子，卵囊与子孢子密度呈线性关系（r ~ 2 = 0.73）。每个卵囊的子孢子产量不受卵囊密度的影响，几乎所有的卵囊感染都会导致唾液腺的子孢子感染。本定量研究表明，在实验室感染的A。冈比亚传播是一个效率低下的过程，动合子是影响病媒传染性概率的关键过渡阶段。
The population dynamics of cultured Plasmodium falciparum parasites was examined during their sporogonic development in Anopheles gambiae mosquitoes. Estimates of absolute denisities were determined for each life stage, and life tables were contructed for each of 38 experimental infections. Macrogametocyte and ookinete mortalities contributed equally to the overall mortality. On average, there was a 40-fold decrease in parasite numbers in the transition from the macrogametocyte to the ookinete stage, a 69-fold decrease in the transition from ookinete to oocyst stages, and a total net decrease in parasite numbers from macrogametocyte to oocyst stage of 2,754-fold (i.e., multiplicative). There was no relationship between macrogametocyte and ookinete densities due to the inherent variability in fertility among different gametocyte cultures. There was a curvilinear relationship (r2 = 0.66) between ookinete and oocyst densities. Above a threshold of about 30 ookinetes/mosquito, the oocyst yield per ookinete became increasingly greater with increasing ookinete density. There was a linear relationship (r2 = 0.73) between oocyst and sporozoite densities, with an average of 663 salivary gland sporozoites produced per oocyst. Sporozoite production per oocyst was not affected by oocyst density and virtually all oocyst infections resulted in sporozoite infections of the salivery glands. This quantitative study indicates that the sporogony of cultured P. falciparum in laboratory-infected A. gambiae is an inefficient process and that the ookinete is the key transitional stage affecting the probability of vector infectivity.