The study of a single BGC823 cell using Fourier transform infrared microspectroscopic imaging.
The study of a single BGC823 cell using Fourier transform infrared microspectroscopic imaging.
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DOI：
10.1016/j.saa.2011.05.031
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发表时间：
2011-09
期刊：
影响因子：
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通讯作者：
Xin Wang;Z. Qi;Shengyi Wang;Gang Liu;H. Gao;Yangchao Tian
中科院分区：
文献类型：
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作者：
Xin Wang;Z. Qi;Shengyi Wang;Gang Liu;H. Gao;Yangchao Tian
为了在细胞和亚细胞水平上研究胃癌，通过配备焦平面阵列（FPA）探测器的红外显微镜对单个人胃腺癌BGC823细胞进行了研究。光谱显示 BGC823 细胞的细胞核和内质网 (ER) 之间的差异。与 ER 处相比，vasPO2− 的峰值在细胞核处移动到更高的波数。细胞核2954cm−1/2922cm−1(CH3/CH2)和1088cm−1/1539cm−1(DNA/amide II)的高度比显着高于ER。此外，化学图像揭示了单个BGC823细胞的脂质、蛋白质和DNA的强度分布，在细胞核区域观察到蛋白质和DNA的强烈吸收，而在细胞的ER区域发现脂质的强烈吸收。傅里叶变换红外(FTIR)显微光谱成像结果表明，在亚细胞水平上对单个胃癌细胞进行研究有利于更多地了解胃癌，对胃癌的研究和诊断具有重要意义。该结果还表明，FPA 是单细胞研究中的有用工具，并且可能是研究和诊断胃癌的有力工具。
In order to investigate gastric cancer at cellular and sub-cellular level, a single human gastric adenocarcinoma BGC823 cell was studied by an infrared microscope equipped with a focal plane array (FPA) detector. The spectra showed difference between the nucleus and the endoplasmic reticulum (ER) of the BGC823 cell. The peak of vasPO2−was shifted to a higher wavenumber at the nucleus compared with that at the ER. The height ratios of 2954cm−1/2922cm−1(CH3/CH2) and 1088cm−1/1539cm−1(DNA/amide II) of the nucleus were significantly higher than those of the ER. Furthermore, chemical images reveal the intensity distributions of lipids, proteins and DNA of the single BGC823 cell, and the intense absorptions of proteins and DNA were observed in the nuclear region of the cell while the intense absorption of lipids was found in the ER region of the cell. The Fourier transform infrared (FTIR) microspectroscopic imaging result indicates the study of the single gastric cancer cell at sub-cellular level can be beneficial for knowing gastric cancer more which will be of great importance for the study and diagnosis of gastric cancer. The result also suggests that FPA is a useful tool in the study of a single cell and may be a powerful tool for study and diagnosis of gastric cancer.