SAPK10-Mediated Phosphorylation on WRKY72 Releases Its Suppression on Jasmonic Acid Biosynthesis and Bacterial Blight Resistance
SAPK10-Mediated Phosphorylation on WRKY72 Releases Its Suppression on Jasmonic Acid Biosynthesis and Bacterial Blight Resistance
复制标题
SAPK10 介导的 WRKY72 磷酸化释放了对茉莉酸生物合成和白叶枯病抗性的抑制。
DOI：
10.1016/j.isci.2019.06.009
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发表时间：
2019-06-28
期刊：
影响因子：
5.8
通讯作者：
Zhang, Jian
中科院分区：
文献类型：
--
作者：
Hou, Yuxuan;Wang, Yifeng;Zhang, Jian
细菌性枯萎病是由黄单胞菌致病变种（Xanthomonasplantypv.）稻曲病是严重威胁水稻产量的毁灭性病害。在这里，我们报告了一个“SAPK 10-WRKY 72-AOS 1”模块的鉴定，通过该模块Xoo感染刺激茉莉酸（JA）生物合成的抑制，导致Xoo易感性。WRKY 72直接与JA生物合成基因AOS 1启动子中的W-box结合，通过诱导目标位点的DNA超甲基化抑制其转录，最终导致内源JA水平降低和Xoo易感性升高。脱落酸（阿坝）诱导的SnRK 2型激酶SAPK 10磷酸化WRKY 72的Thr 129。SAPK 10介导的磷酸化削弱WRKY 72的DNA结合能力，并释放其对AOS 1和JA生物合成的抑制。我们的工作突出了病原刺激如何导致植物易感性的模块，以及ABA-JA相互作用的翻译后修饰和表观遗传调控机制涉及的潜在途径。
Bacterial blight caused by the infection of Xanthomonas oryzae pv. oryzae (Xoo) is a devastating disease that severely challenges the yield of rice. Here, we report the identification of a "SAPK10-WRKY72-AOS1" module, through which Xoo infection stimulates the suppression of jasmonic add (JA) biosynthesis to cause Xoo susceptibility. WRKY72 directly binds to the W-box in the promoter of JA biosynthesis gene AOS1 and represses its transcription by inducing DNA hypermethylation on the target site, which finally led to lower endogenous JA level and higher Xoo susceptibility. Abscisic add (ABA)-inducible SnRK2-type kinase SAPK10 phosphorylates WRKY72 at Thr 129. The SAPK10-mediated phosphorylation impairs the DNA-binding ability of WRKY72 and releases its suppression on AOS1 and JA biosynthesis. Our work highlights a module of how pathogen stimuli lead to plant susceptibility, as well as a potential pathway for ABA-JA interplay with post-translational modification and epigenetic regulation mechanism involved.