Characterization of oxygenated metabolites of ginsenoside Rb1 in plasma and urine of rat.
Characterization of oxygenated metabolites of ginsenoside Rb1 in plasma and urine of rat.
复制标题
DOI：
10.1021/acs.jafc.5b00710
复制
发表时间：
2015-03
影响因子：
6.1
通讯作者：
Jing-Rong Wang;Lee-Fong Yau;T. Tong;Qitong Feng;Li-Ping Bai;Jing Ma;Ming Hu;Liang Liu;Zhihong Jiang
中科院分区：
文献类型：
--
作者：
Jing-Rong Wang;Lee-Fong Yau;T. Tong;Qitong Feng;Li-Ping Bai;Jing Ma;Ming Hu;Liang Liu;Zhihong Jiang
氧化代谢物已被认为是主要的循环代谢物的皂苷。本研究以化学合成的化合物quinquenoside L16为对照，对大鼠静脉给药后血浆和尿液中10个含氧代谢产物进行了表征（M1和M2）、notoA（M3）、NotoV（M4和M7）、环氧丙烷Rb1（M5和M9）、三七酮K（M6）和三七酮C（M8和M10），其中9种首次被检测为体内代谢产物。口服给药后，观察到Rb1、M3、M4和M7作为主要循环代谢产物，并在大鼠血流中存在24 h。这些循环代谢物的确切化学结构的表征可以大大有助于我们理解食用人参产品后人参皂苷的化学暴露，并为解释人参产品的多种生物活性提供有价值的信息。
Oxygenated metabolites have been suggested as the major circulating metabolites of ginsenosides. In the current study, 10 oxygenated metabolites of ginsenoside Rb1 in plasma and urine of rat following iv dose were characterized by comparison with chemically synthesized authentic compounds as quinquenoside L16 (M1 and M2), notoginsenoside A (M3), ginsenoside V (M4 and M7), epoxyginsenoside Rb1 (M5 and M9), notoginsenoside K (M6), and notoginsenoside C (M8 and M10), 9 of which were detected as in vivo metabolites for the first time. After oral administration of ginsenoside Rb1, M3, M4, and M7 were observed as major circulating metabolites and presented in the bloodstream of rat for 24 h. Characterization of the exact chemical structures of these circulating metabolites could contribute greatly to our understanding of chemical exposure of ginsenosides after consumption of ginseng products and provide valuable information for explaining multiple bioactivities of ginseng products.