Thymocyte apoptosis by T-2 toxin in vivo in mice is independent of Fas/Fas ligand system
Thymocyte apoptosis by T-2 toxin in vivo in mice is independent of Fas/Fas ligand system
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DOI：
10.1271/bbb.64.210
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发表时间：
2000-01-01
影响因子：
1.6
通讯作者：
Sakato, N
中科院分区：
文献类型：
--
作者：
Alam, MM;Nagase, M;Sakato, N
为探讨T-2毒素（T-2）介导的胸腺细胞凋亡是否与Fas/Fas配体（FasL）通路有关，我们采用Fas和FasL蛋白功能缺陷的lpr/lpr（lpr）和gld/gld（gld）小鼠，通过凝胶电泳DNA片段化和流式细胞仪检测凋亡细胞百分率，在接受T-2的Ipr和gld小鼠中胸腺细胞凋亡的水平表明，Ipr和gld小鼠都经历了与相应的野生型小鼠（+/+）基本上相同程度的凋亡。这些结果有力地表明，T-2诱导的小鼠胸腺细胞凋亡在体内是独立的Fas/FasL途径。
To find whether Fas/Fas ligand (FasL) pathway is involved in T-2 toxin (T-2)-mediated thymocyte apoptosis, we used lpr/lpr (lpr) and gld/gld (gld) mice, whose Fas and FasL proteins, respectively, are functionally deficient, Based on the DNA fragmentation profile in gel electrophoresis and measurement of apoptotic cell percent by how cytometry, the levels of thymocyte apoptosis in Ipr and gld mice that had received T-2 showed that both lpr and gld mice had undergone apoptosis essentially to the same magnitude as those of corresponding wild type mice (+/+). These results strongly suggest that T-2-induced thymocyte apoptosis in vivo in mice is independent of the Fas/FasL pathway.