p37 is a p97 adaptor required for Golgi and ER biogenesis in interphase and at the end of mitosis.
p37 is a p97 adaptor required for Golgi and ER biogenesis in interphase and at the end of mitosis.
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DOI：
10.1016/j.devcel.2006.10.016
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发表时间：
2006-12
影响因子：
11.8
通讯作者：
K. Uchiyama;G. Totsukawa;M. Puhka;Y. Kaneko;E. Jokitalo;I. Dreveny;F. Beuron;Xiaodong Zhang;P. Freemont;Hisao Kondo
中科院分区：
文献类型：
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作者：
K. Uchiyama;G. Totsukawa;M. Puhka;Y. Kaneko;E. Jokitalo;I. Dreveny;F. Beuron;Xiaodong Zhang;P. Freemont;Hisao Kondo
我们以前报道过p97/p47辅助的膜融合对于有丝分裂结束时细胞器的重新组装是重要的，但对于它们在间期的维持并不重要。我们现在已经确定了一个p97衔接蛋白，p37，它形成一个复合物与p97在胞质溶胶和本地化的高尔基体和ER。siRNA实验表明，p37是高尔基体和ER生物合成所必需的。注射抗p37抗体到细胞在不同的细胞周期阶段表明，p37在高尔基体和ER的维护期间的间期，以及在有丝分裂结束时，在他们的重新组装起着重要的作用。在体外高尔基体重组测定中，p97/p37复合物具有膜融合活性。与p97/p47通路相反，该通路需要p115-GM 130栓系和SNARE GS15，但不需要syntaxin 5。有趣的是，虽然也需要VCIP 135，但其去泛素化活性对于p97/p37介导的活性是不必要的。
We previously reported that p97/p47-assisted membrane fusion is important for the reassembly of organelles at the end of mitosis, but not for their maintenance during interphase. We have now identified a p97 adaptor protein, p37, which forms a complex with p97 in the cytosol and localizes to the Golgi and ER. siRNA experiments revealed that p37 is required for Golgi and ER biogenesis. Injection of anti-p37 antibodies into cells at different cell cycle stages showed that p37 plays an important role in both Golgi and ER maintenance during interphase as well as in their reassembly at the end of mitosis. In an in vitro Golgi reassembly assay, the p97/p37 complex has membrane fusion activity. In contrast to the p97/p47 pathway, this pathway requires p115-GM130 tethering and SNARE GS15, but not syntaxin5. Interestingly, although VCIP135 is also required, its deubiquitinating activity is unnecessary for p97/p37-mediated activities.