Fungal speciation using quantitative polymerase chain reaction (QPCR) in patients with and without chronic rhinosinusitis
Fungal speciation using quantitative polymerase chain reaction (QPCR) in patients with and without chronic rhinosinusitis
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DOI：
10.1097/01.mlg.0000225896.91392.6a
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发表时间：
2006-08-01
期刊：
影响因子：
2.6
通讯作者：
Vesper, Stephen
中科院分区：
文献类型：
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作者：
Murr, Andrew H.;Goldberg, Andrew N.;Vesper, Stephen
目的/假设：本研究的目的是使用内窥镜引导刷采样技术和聚合酶链反应实验室处理鼻粘液来确定中鼻道的真菌学；比较患有鼻窦疾病的患者与没有鼻窦疾病的受试者的中鼻道真菌学；比较患有和不患有鼻窦炎的患者对两种标准化生活质量调查表的反应；并确定中鼻道中真菌的存在是否与这些数据集的响应相关。研究设计：作者进行了一项单盲、前瞻性、横断面研究。方法：患有鼻窦疾病的患者和没有鼻窦疾病的对照组参加了研究。受试者完成了针对特定疾病的经验证的鼻腔结果测试调查 (SNOT-20)，还完成了通用的经过验证的医疗结果简表 36 调查 (SF-36)。在内窥镜引导下从中鼻道刷取鼻粘液样本。对获得的样品进行真菌特异性定量聚合酶链反应 (QPCR)，以在实验室中鉴定 82 种不同真菌中的一种。统计分析用于对回收的真菌 DNA 进行分类，并将这些信息与结果调查进行交叉引用。结果：研究中鼻窦疾病患者的真菌恢复率为 45.9%，对照组为 45.9%。慢性鼻窦炎患者的平均 SNOT-20 评分为 1.80，而对照组平均评分为 0.77 (P < .0001)。 SF-36 数据同样显示患病人群和对照人群之间存在统计学显着差异，对照人群的平均得分为 80.37，慢性鼻窦炎患者的平均得分为 69.35，P 值为 0.02。然而，是否存在回收的真菌、回收的真菌的类型或真菌对生活质量调查结果的可能影响并不能归因于统计显着性。结论：采用QPCR技术，慢性鼻-鼻窦炎患者和非慢性鼻-鼻窦炎对照人群中鼻道真菌回收率为45.9%。没有证明与真菌种类或存在有直接因果关系；然而，根据这些数据和其他报告的趋势，提出了未来研究的物种分组。针对特定疾病的结果调查显示两组之间存在统计学上的显着差异。
Objectives/Hypothesis: The objectives of this study were to determine the mycology of the middle meatus using an endoscopically guided brush sampling technique and polymerase chain reaction laboratory processing of nasal mucous; to compare the mycology of the middle meatus in patients with sinus disease with subjects without sinus disease; to compare the responses on two standardized quality-of-life survey forms between patients with and without sinusitis; and to determine whether the presence of fungi in the middle meatus correlates with responses on these data sets. Study Design: The authors conducted a single-blind, prospective, cross-sectional study. Methods: Patients with sinus disease and a control group without sinus disease were enrolled in the study. A disease-specific, validated Sinonasal Outcomes Test survey (SNOT-20) was completed by the subjects and a generalized validated Medical Outcomes Short Form 36 Survey (SF-36) was also completed. An endoscopically guided brush sampling of nasal mucous was obtained from the middle meatus. Fungal specific quantitative polymerase chain reaction (QPCR) was performed on the obtained sample to identify one of 82 different species of fungus in the laboratory. Statistical analysis was used to categorize the recovered fungal DNA and to crossreference this information with the outcomes surveys. Results: The fungal recovery rate in the study was 45.9% in patients with sinus disease and 45.9% in control subjects. Patients with chronic rhinosinusitis had a mean SNOT-20 score of 1.80 versus the control group mean score of 0.77 (P < .0001). SF-36 data similarly showed a statistically significant difference between diseased and control populations with controls scoring a mean of 80.37 and patients with chronic rhinosinusitis scoring a mean of 69.35 for a P value of .02. However, no statistical significance could be ascribed to the presence or absence of fungi recovered, the type of fungi recovered, or the possible impact of fungi on the quality-of-life survey results. Conclusion: The recovery rate of fungi from the middle meatus of patients with chronic rhinosinusitis and a control population without chronic rhinosinusitis is 45.9% using QPCR techniques. No direct causation with regard to fungal species or presence was proven; however, a species grouping for future studies is proposed based on trends in this data and other reports. Disease-specific outcomes surveys revealed a statistically significant difference between the two groups.