Lack of correlation between chemokine receptor and Th1/Th2 cytokine expression by individual memory T cells
Lack of correlation between chemokine receptor and Th1/Th2 cytokine expression by individual memory T cells
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DOI：
10.1093/intimm/12.12.1659
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发表时间：
2000-12-01
影响因子：
4.4
通讯作者：
Lipsky, PE
中科院分区：
文献类型：
--
作者：
Nanki, T;Lipsky, PE
趋化因子和趋化因子受体的相互作用可能在白细胞向特定免疫反应部位迁移中发挥重要作用。最近有报道，CXC趋化因子受体(CXCR)3和CC趋化因子受体(CCR)5在T(H)1细胞上优先表达，CCR3和CCR4在T(H)2细胞上优先表达。为了研究T-h亚群在体内的趋化因子受体的表达，我们采用单细胞逆转录聚合酶链式反应(RT-PCR)的方法，分析了短期刺激后单个外周血中细胞因子(IL-2、IL-4和干扰素-γ)和趋化因子受体(CXCR3、CXCR4、CCR3、CCR4和CCR5)的mRNA表达。体外分析表明，正常外周血中T(H)1和T(H)2细胞表达趋化因子受体基因的细胞频率无显著差异。为了评估体内刺激的潜在作用，我们还分析了未刺激的类风湿性关节炎滑膜CD4(+)记忆T细胞。单个滑膜T细胞表达CXCR3、CXCR4、CCR3和CCR5，但所有RA患者T(H)1和非T(H)1细胞趋化因子受体基因表达频率无明显差异。这些数据表明，趋化因子受体的表达不能识别产生Th定义细胞因子的单个记忆T细胞，因此趋化因子受体的表达不能作为体内T(H)1或T(H)2细胞的标志。
Chemokine and chemokine receptor interactions may have important roles in leukocyte migration to specific immune reaction sites. Recently, it has been reported that CXC chemokine receptor (CXCR) 3 and CC chemokine receptor (CCR) 5 were preferentially expressed on T(h)1 cells, and CCR3 and CCR4 were preferentially expressed on T(h)2 cells. To investigate chemokine receptor expression by T-h subsets in vivo, we analyzed cytokine (IL-2, IL-4 and IFN-gamma) and chemokine receptor (CXCR3, CXCR4, CCR3, CCR4 and CCR5) mRNA expression by individual peripheral CD4(+) memory T cells after short-term stimulation, employing a single-cell RT-PCR method. This ex vivo analysis shows that the frequencies of cells expressing chemokine receptor mRNA were not significantly different between T(h)1 and T(h)2 cells in normal peripheral blood. To assess a potential role of in vivo stimulation, we also analyzed unstimulated rheumatoid arthritis synovial CD4(+) memory T cells. CXCR3, CXCR4, CCR3 and CCR5 expression was detected by individual synovial T cells, but the frequencies of chemokine receptor mRNA were not clearly different between T(h)1 and non-T(h)1 cells defined by expression of IFN-gamma or lymphotoxin-a mRNA in all RA patients. These data suggest that chemokine receptor expression does not identify individual memory T cells producing Th-defining cytokines and therefore chemokine receptor expression cannot be a marker for T(h)1 or T(h)2 cells in vivo.