BONE CELL FORMATION AND SURVIVAL IN H3-THYMIDINE-LABELED CHICKS UNDER VARIOUS CONDITIONS
BONE CELL FORMATION AND SURVIVAL IN H3-THYMIDINE-LABELED CHICKS UNDER VARIOUS CONDITIONS
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DOI：
10.1002/ar.1091450303
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发表时间：
1963-01-01
影响因子：
--
通讯作者：
MIGICOVSKY, BB
中科院分区：
文献类型：
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作者：
BELANGER, LF;MIGICOVSKY, BB
用H3-胸腺嘧啶核苷标记了三周龄雏鸡的分裂细胞，并在不同的营养和激素条件下，通过放射自显影对分裂细胞进行了观察。2小时后标记大部分原始细胞(间充质细胞、成纤维细胞、软骨母细胞、造血祖细胞)。标记破骨细胞和成骨细胞在1天和2天后大量出现，此后数量减少。标记的骨细胞在第二天和第四天出现，但很少在之后出现。软骨病患者骨细胞成熟延迟。与脊椎病组相比，维生素D治疗组的鸟的破骨细胞显示出更大比例的标记核。另外，在三组雏鸡中，破骨细胞中标记核的出现和消失的比率是恒定的。破骨细胞在它们的生命周期中，随着年老的细胞核退化，通过吞并年轻的细胞核来更新它们的细胞核。
Dividing cells have been labeled in three week old chicks with H3-thymidine and observed through radioautography at different time intervals and also under varied nutritional and hormonal conditions. After two hours mostly primitive cells were labeled (mesenchymal cells, fibroblasts, chondroblasts, hemopoietic precursors). Labeled osteoclasts and osteoblasts were abundant after one and two days and their number decreased thereafter. Labeled osteocytes were present at two and four days but rarely thereafter. Osteocyte maturation was delayed in rickets. The osteoclasts showed a larger proportion of labeled nuclei in the Vitamin-D-treated birds as compared to the rachitic group. Otherwise the rate of appearance and disappearance of labeled nuclei in osteoclasts was constant in the three groups of chicks. Osteoclasts during their life span, renew their nuclei by annexation of young ones as older nuclei degenerate.