Molecular mechanism of Wnt signal pathway in multiple myeloma cell line H929 cell autophagy.
Molecular mechanism of Wnt signal pathway in multiple myeloma cell line H929 cell autophagy.
复制标题
多发性骨髓瘤细胞系H929细胞自噬Wnt信号通路的分子机制
DOI：
10.26355/eurrev_201806_15152
复制
发表时间：
2018
影响因子：
3.3
通讯作者：
Yang
中科院分区：
文献类型：
--
作者：
Liu Ys;Liu Xb;Qiu Yy;T. Lan;Yang
目的
多发性骨髓瘤的发病机制尚不清楚。细胞自噬异常与各种骨科疾病尤其是多发性骨髓瘤密切相关。因此，本研究探讨了细胞自噬异常在多发性骨髓瘤发生中的机制及其临床意义。
材料和方法
以多发性骨髓瘤细胞系H929为模型，用紫外线照射细胞。Western blot检测H929细胞自噬、自噬分子表达水平及Wnt等自噬信号通路的激活情况。使用自噬激活剂，增强H929细胞的自噬，然后定量自噬分子表达和信号途径（例如Wnt激活）。用Wnt信号通路的激动剂或拮抗剂处理H929细胞，然后测量自噬分子和Wnt通路活化。分析Wnt信号通路和细胞自噬与多发性骨髓瘤发生的相关性。
结果
紫外线照射诱导多发性骨髓瘤细胞H929自噬和Wnt信号通路激活。Wnt信号通路抑制剂可抑制紫外线诱导的H929细胞自噬。然而，Wnt信号通路的过表达增强了UV介导的H929细胞自噬。Wnt激活状态与自噬水平呈正相关。
结论
紫外线照射可以诱导多发性骨髓瘤细胞自噬，提示细胞自噬的管理可能是多发性骨髓瘤的一种可能的治疗方法。
OBJECTIVE
Pathogenic mechanism of multiple myeloma is still unclear yet. Abnormality in cell autophagy is closely correlated with various orthopedic diseases especially multiple myeloma. Therefore, this study investigated the mechanism of cell autophagy abnormality in multiple myeloma occurrence and clinical implications.
MATERIALS AND METHODS
Using multiple myeloma cell line H929 as the model, cells were treated with UV irradiation. Western blot was used to measure the autophagy of H929 cell, expression level of autophagy molecules and activation of autophagy signal pathway such as Wnt. Using autophagy activator, H929 cell autophagy was potentiated, followed by quantification of autophagy molecular expression and signal pathway such as Wnt activation. Agonist or antagonist of Wnt signal pathway was used to treat H929 cells followed by measuring autophagy molecules and Wnt pathway activation. The correlation between Wnt signal pathway or cell autophagy and occurrence of multiple myeloma was analyzed.
RESULTS
UV irradiation treatment on multiple myeloma cell line H929 induced autophagy and Wnt signal pathway activation. The inhibitor of Wnt signal pathway suppressed UV-induced H929 cell autophagy. However, over-expression of Wnt signal pathway enhanced UV-mediated autophagy of H929 cells. The condition of Wnt activation and autophagy level were positively correlated.
CONCLUSIONS
UV irradiation can induce autophagy of multiple myeloma cells, suggesting that management of cell autophagy might be one possible treatment for multiple myeloma.