Pulp mill wastewater sediment reveals novel methanogenic and cellulolytic populations.
Pulp mill wastewater sediment reveals novel methanogenic and cellulolytic populations.
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DOI：
10.1016/j.watres.2012.10.038
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发表时间：
2013-02
期刊：
影响因子：
12.8
通讯作者：
Chunyu Yang;Wei Wang;Miaofen Du;Chunfang Li;Cuiqing Ma;P. Xu
中科院分区：
文献类型：
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作者：
Chunyu Yang;Wei Wang;Miaofen Du;Chunfang Li;Cuiqing Ma;P. Xu
麦草制浆废水具有高碱度、高COD污染负荷的特点。对废旧纸浆厂废水蓄水池中自然发育的微生物群落进行了调查。由于自然蒸发和木质纤维素的大量沉积，废水底泥中有机物和钠离子浓度较高，但氯化物和碳酸盐浓度较低。研究了厌氧菌群中的微生物区系，包括产甲烷菌和纤维素分解菌。所有的古细菌序列归入盐杆菌科和产甲烷古细菌2个类群，在Eurya chaeota门。在产甲烷群落中，针对甲烷菌属新种或微小甲烷菌目新属的甲基辅酶M和还原酶A(MCRA)基因进行系统发育分析。甲烷微生物菌的优势表明，该系统中的甲烷生成可能是由水遗传营养途径驱动的。作为产甲烷的重要初级发酵剂，GHF48酶的纤维素酶降解群落主要由新的梭状芽胞杆菌纤维素酶基因组成。这种极端沉积物中的新型缺氧功能体提供了提高盐碱废水缺氧处理效率的可能性，并有利于生物质转化和生物燃料生产过程。
Pulp mill wastewater generated from wheat straw is characterized as high alkalinity and very high COD pollution load. A naturally developed microbial community in a pulp mill wastewater storage pool that had been disused were investigated in this study. Owing to natural evaporation and a huge amount of lignocellulose's deposition, the wastewater sediment contains high concentrations of organic matters and sodium ions, but low concentrations of chloride and carbonate. The microbiota inhabiting especially anaerobic community, including methanogenic arhcaea and cellulolytic species, was studied. All archaeal sequences fall into 2 clusters of family Halobacteriaceae and methanogenic archaeon in the phylum Euryarchaeota. In the methanogenic community, phylogenetic analysis of methyl coenzyme M reductase A (mcrA) genes targeted to novel species in genus Methanoculleus or novel genus of order Methanomicrobiales. The predominance of Methanomicrobiales suggests that methanogenesis in this system might be driven by the hydrogenotrophic pathway. As the important primary fermenter for methane production, the cellulolytic community of enzyme GHF48 was found to be dominated by narrower breadth of novel clostridial cellulase genes. Novel anoxic functional members in such extreme sediment provide the possibility of enhancing the efficiency of anoxic treatment of saline and alkaline wastewaters, as well as benefiting to the biomass transformation and biofuel production processes.