Expression of anion exchanger 1 is associated with tumor progress in human gastric cancer
Expression of anion exchanger 1 is associated with tumor progress in human gastric cancer
复制标题
阴离子交换剂1的表达与人胃癌的肿瘤进展相关。
DOI：
10.1007/s00432-009-0573-9
复制
发表时间：
2009-10-01
影响因子：
3.6
通讯作者：
Fu, Guo-Hui
中科院分区：
文献类型：
--
作者：
Xu, Wei-Qing;Song, Ling-Jun;Fu, Guo-Hui
阴离子交换蛋白1（AE 1）是一种跨膜糖蛋白，在红细胞膜上大量表达，介导Cl-和HCO 3（-）的电中性交换。我们曾报道AE 1蛋白在胃癌和结肠癌中意外表达，并参与癌细胞的癌变过程。本研究采用免疫组化方法检测胃癌组织中AE 1蛋白的表达，探讨AE 1蛋白在胃癌中的表达及其临床意义。本文检测了286例早期胃癌和进展期胃癌组织中AE 1的表达。采用卡方检验和t检验分析AE 1表达与患者年龄、肿瘤大小、部位及亚型、表达频率、生存期、淋巴结转移等临床病理参数的关系。AE 1在胃癌中的表达与胃癌的病理分级无关。AE 1在胃窦癌中的表达率最高（P = 0.034）。AE 1表达与肿瘤体积大、浸润深、生存期短、无淋巴结转移有关。在弥漫型胃癌癌旁组织中，AE 1的表达频率明显高于弥漫型胃癌（P = 0.011），提示AE 1的表达与胃癌的发生、发展密切相关，有助于提高胃癌的分型和治疗水平。
Anion exchanger 1 (AE1) is a transmembrane glycoprotein which is abundantly expressed in erythrocyte plasma membrane and mediates the electroneutral exchange of Cl- and HCO3 (-). We previously reported that the AE1 protein was unexpectedly expressed in the gastric and colonic cancer and take part in the carcinogenesis of the cancer cells. The aim of the present study is to determine the potential clinical implications of AE1 expression in gastric carcinoma.Immunohistochemistry assay was used to determine the expression of AE1 protein. The expression of AE1 in normal and malignant tissues from 286 patients with early and advanced gastric carcinoma was examined. The correlations of AE1 expression with clinicopathological parameters, including age, tumor size, location and subtypes, expression frequency, survival period and lymph metastasis were assessed by Chi-squared test and t test analysis.AE1 immunoreactivity was negative in normal gastric tissue. Positive immunostaining of AE1 was detected in gastric carcinoma regardless of the location. AE1 was most frequently expressed in the gastric antrum carcinoma compared with gastric body cancer (P = 0.034). Expression of AE1 was significantly associated with bigger tumor size, deeper invasion, shorter survival period, and non-lymph metastasis. In para-cancer tissues of intestinal-type gastric cancer, the expression frequency of AE1 was higher than that in diffuse-type (P = 0.011).The results showed a strong association of AE1 expression with the onset and progression of the gastric cancer and that may be helpful for improving the tumor classification and the treatment of cancer.