The Use of Silk as a Scaffold for Mature, Sustainable Unilocular Adipose 3D Tissue Engineered Systems.
The Use of Silk as a Scaffold for Mature, Sustainable Unilocular Adipose 3D Tissue Engineered Systems.
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DOI：
10.1002/adhm.201600211
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发表时间：
2016-07
影响因子：
10
通讯作者：
Kaplan DL
中科院分区：
文献类型：
--
作者：
Abbott RD;Wang RY;Reagan MR;Chen Y;Borowsky FE;Zieba A;Marra KG;Rubin JP;Ghobrial IM;Kaplan DL
迫切需要在体外监测生理上相关的、可持续的人类脂肪组织，以获得对代谢性疾病的新见解。为了支持长期培养，建立了一种3D丝质支架辅助培养系统，将成熟的单眼脂肪细胞与从少量液化脂肪样品中获得的前脂肪细胞、内皮细胞和平滑肌细胞进行体外共培养。如果没有丝素支架，脂肪组织外植体因其脆性而不能长期培养(3个月)。除了对炎性刺激的反应性外，对介质成分的调整还用于调节脂质代谢和增殖。有趣的是，观察到了患者对肿瘤坏死因子α刺激的特异性反应，为未来患者特异性疾病建模提供了概念转换技术的验证。综上所述，我们的新型3D支架辅助方法是从少量吸脂物中建立生理上相关的、可持续的人类脂肪组织系统所必需的，这使得这种方法对于新陈代谢、脂肪因子驱动的疾病以及脂肪细胞的作用现在才被发现的其他疾病的研究具有重要价值。
There is a critical need for monitoring physiologically relevant, sustainable, human adipose tissues in vitro to gain new insights into metabolic diseases. To support long term culture, a 3D silk scaffold assisted culture system was developed that maintained mature unilocular adipocytes ex vivo in co-culture with pre-adipocytes, endothelial cells, and smooth muscle cells obtained from small volumes of liquefied adipose samples. Without the silk scaffold, adipose tissue explants could not be sustained in long term culture (3 months) due to their fragility. Adjustments to media components were used to tune lipid metabolism and proliferation, in addition to responsiveness to an inflammatory stimulus. Interestingly, patient specific responses to TNFα stimulation were observed, providing a proof of concept translational technique for patient specific disease modeling in the future. In summary, our novel 3D scaffold assisted approach is required for establishing physiologically relevant, sustainable, human adipose tissue systems from small volumes of lipoaspirate, making this methodology of great value to studies of metabolism, adipokine-driven diseases, and other diseases where the roles of adipocytes are only now becoming uncovered.