Biophoton detection as a novel technique for cancer imaging
Biophoton detection as a novel technique for cancer imaging
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DOI：
10.1111/j.1349-7006.2004.tb03325.x
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发表时间：
2004-08-01
期刊：
影响因子：
5.7
通讯作者：
Ohuchi, N
中科院分区：
文献类型：
--
作者：
Takeda, M;Kobayashi, M;Ohuchi, N
生物光子发射被定义为任何生命系统由于其代谢活动而辐射出的极弱的光，没有激发或增强。采用高灵敏度、超低噪声的CCD摄像系统测量小鼠移植肿瘤的光子图像。本研究使用的细胞系为AH109A、TE4和TE9。在癌细胞移植后1周测量每个肿瘤的光子图像，以估计第1周肿瘤的大小和光子强度。在第2周和第3周还测量了一些，以比较生物光子分布与组织学结果。我们首次实现了肿瘤生长过程中的连续光子成像。显微观察结果与生物光子强度的比较表明，生物光子发射强度反映了肿瘤组织的活力。不同细胞系第1周肿瘤大小不同，肿瘤的光子强度与第1周肿瘤大小相关（相关系数0.73）。这种非侵入性和简单的技术有可能被用作光学活检来检测肿瘤的生存能力。
Biophoton emission is defined as extremely weak light that is radiated from any living system due to its metabolic activities, without excitation or enhancement. We measured biophoton images of tumors transplanted in mice with a highly sensitive and ultra-low noise CCD camera system. Cell lines employed for this study were AH109A, TE4 and TE9. Biophoton images of each tumor were measured 1 week after carcinoma cell transplantation to estimate the tumor size at week 1 and the biophoton intensity. Some were also measured at 2 and 3 weeks to compare the biophoton distribution with histological findings. We achieved sequential biophoton imaging during tumor growth for the first time. Comparison of microscopic findings and biophoton intensity suggested that the intensity of biophoton emission reflects the viability of the tumor tissue. The size at week 1 differed between cell lines, and the biophoton intensity of the tumor was correlated with the tumor size at week 1 (correlation coefficient 0.73). This non-invasive and simple technique has the potential to be used as an optical biopsy to detect tumor viability.