Use of gene fusions to study expression of cysB, the regulatory gene of the cysteine regulon
Use of gene fusions to study expression of cysB, the regulatory gene of the cysteine regulon
复制标题
使用基因融合研究半胱氨酸调节子的调节基因 cysB 的表达
DOI：
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发表时间：
1981
影响因子：
3.2
通讯作者：
D. Hulanicka
中科院分区：
文献类型：
--
作者：
G. Jagura;D. Hulanicka
Strains of escherichia coli were constructed in which the lacZ gene is fused to cysB, the positive regulator gene of the cysteine regulon. The fusion strains were used to study the regulation of the cysB gene by assaying the fused lacZ gene product. The introduction of a cysB allele, either on a plasmid or on an episome to the fusion strains, resulted in the decrease of beta-galactosidase activity. This implies that the cysB gene expression is autoregulated by its own product. The direction of cysB gene transcription was determined to be clockwise.
