Drug resistance & virulence determinants in clinical isolatesof Enterococcus species
Drug resistance & virulence determinants in clinical isolatesof Enterococcus species
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耐药性
DOI：
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发表时间：
2013
期刊：
影响因子：
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通讯作者：
B. Dhanashree
中科院分区：
文献类型：
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作者：
Sanal Fernandes;B. Dhanashree
背景与目的：肠球菌是引起医院感染的主要原因，是全球范围内一个持续存在的临床问题。我们进行了这项研究，以确定肠球菌临床分离株的毒力因子和耐药性。研究方法：对150株临床分离的肠球菌进行了生化鉴定和药敏试验。采用琼脂筛选法测定万古霉素耐药性。溶血素和明胶酶的生产分别使用5%的羊血琼脂和12%的明胶琼脂检测。结果：150株肠球菌中，大肠埃希菌84株（56%）。粪便。51株（34%）E.屎肠球菌，其他肠球菌15株（10%）。123株（82%）分离株产生溶血素，61株（40.6%）分离株产生明胶酶。近50%的分离株表现出高水平的氨基糖苷类耐药（HLAR）。共有13株（8.6%）分离株显示万古霉素耐药，其中11株（7.3%）MIC >8 μg/ml。解释和结论：VRE的存在被认为是低的研究中的分离株。然而，VRE沿着HLAR的发生要求及时准确地定期检测万古霉素耐药性，以识别VRE定植和感染。早期检测VRE和HLAR沿着毒力特性将有助于防止多重耐药肠球菌的建立和传播。
Background & objectives: Enterococci are the leading cause of nosocomial infections, and are thus a persisting clinical problem globally. We undertook this study to determine the virulence factors and the antibiotic resistance in Enterococcus clinical isolates. Methods: One hundred and fifty Enterococcus isolates obtained from various clinical specimens were speciated biochemically and subjected to antibiotic susceptibility testing using Kirby-Bauer disk diffusion method. Resistance to vancomycin was determined by using agar screen method. Haemolysin and gelatinase productions were detected using 5 per cent sheep blood agar and 12 per cent gelatin agar, respectively. Results: Among the 150 Enterococcus isolates, 84 (56%) were E. faecalis. 51(34%) E. faecium, and 15 (10%) were other Enterococcus spp. Haemolysin production was seen among 123 (82%) isolates while 61 (40.6%) isolates produced gelatinase. Nearly 50 per cent of the isolates showed high level aminoglycoside resistance (HLAR). A total of 13 (8.6%) isolates showed vancomycin resistance, of which 11(7.3%) had an MIC >8 μg/ml. Interpretation & conclusions: Presence of VRE was found to be low among the isolates studied. However, occurrence of VRE along with HLAR calls for regular detection of vancomycin resistance promptly and accurately to recognize VRE colonization and infection. Early detection of VRE and HLAR along with their virulence trait will help in preventing the establishment and spread of multidrug resistant Enterococcus species.