Study on the embryonic development of Paralichthys olivaceus
Study on the embryonic development of Paralichthys olivaceus
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DOI：
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发表时间：
2004
影响因子：
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通讯作者：
Tian Yong;Chen Song;Y. An
中科院分区：
文献类型：
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作者：
Tian Yong;Chen Song;Y. An
牙鲆（Paralichthys olivaceus）是我国、朝鲜、俄罗斯和日本等国的重要养殖鱼种。为了进行牙鲆胚胎的冷冻保存研究，明确胚胎发育阶段是非常重要的。另外，对牙鲆胚胎发育的研究将为鱼苗的培育提供支持。本研究对人工孵化的牙鲆胚胎发育进行了研究。在Olympus显微镜下连续观察了牙鲆胚胎在14-16℃海水（盐度29.9）中的发育过程。记录并计算每个发育阶段的总发育时间，并使用Olympus相机拍摄特征图像。从受精到5d后孵化，共记录了29个胚胎发育阶段的特征和时间。拍摄了30张照片，代表了所有发育阶段和鱼苗的特征。受精后0.5h为胚盘形成期，2.5h为四细胞期，10.5h为桑椹胚，17 h为低囊胚期，20 h为早期原肠胚，28.5h为晚期原肠胚，29 h为44 h为关闭囊胚期，28 h为关闭囊胚期。胚胎发育期为30 h 40 m pf、35 h 20 m pf、40 h 10 m pf、49 h 10 m pf、67 h 12 m pf、75 h 10 m pf、90 h 9 m pf、40 h 10 m pf、40 h 10 m pf、40 h 40 m pf、40 h 40 m pf、4-5 h 20 m pf、40 h 15-20 m pf、49 h 10 m pf、67 h 12 m pf、心脏跳动期、75 h 10 m pf、胚胎运动期、孵化前期;受精后190 h卵黄囊被完全吸收，进入变态期。
Japanese flounder (Paralichthys olivaceus), which naturally inhabits the seas of China, Korea, Russia and Japan, has become one of the most important cultivated species. In order to do cryopreservation research of the Japanese flounder embryos, it is important to define definitely embryo developmental stage. In addition, study of Japanese flounder embryo development will provide support for the fry breeding. In the present study, embryonic development of Japanese flounder cultured in hatchery was studied. Japanese flounder embryos were incubated in sea water (salinity: 29.9) at 14-16℃ and the course of embryonic development was observed consecutively under Olympus microscope. The total development time of every development stage was noted and calculated and the characteristic images were taken using Olympus camera. The characteristics and time of 29 embryonic development stages were noted from the fertilization to the hatching after 5 d. 30 photographs, representing the characters of all the development stages and fry, were taken. The time-table of major embryonic development stages of Japanese flounder was as follows: blastodisc forming stage at 0.5 h post fertilization (pf); four cell stage at 2.5 h pf; morula at 10.5 h pf; low blastula stage at 17 h pf; early gastrula stage at 20 h 10 m pf; late gastrula at 28.5 h pf; blastopore closing stage at 29 h 44 m pf; nurula stage at 30 h 40 m pf; 4-5 somite stage at 35 h 20 m pf; 15-20 somite stage at 40 h 10 m pf; tail bud stage at 49 h 10 m pf; heart beating stage at 67 h 12 m pf; embryo movement at 75 h 10 m pf; pre-hatching stage at 90 h 9 m pf; hatching stage at 93 h pf. Yolk sac was completely absorbed 190 h after fertilization and then entered metamorphosis stage.