Effects of progesterone on the oviductal epithelium in estrogen-primed prepubertal beagles: light and electron microscopic observations.
Effects of progesterone on the oviductal epithelium in estrogen-primed prepubertal beagles: light and electron microscopic observations.
复制标题
黄体酮对雌激素引发的青春期前比格犬输卵管上皮的影响：光和电子显微镜观察。
DOI：
10.1002/aja.1001690107
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Gorell,TA
中科院分区：
文献类型：
--
作者：
Sawyer,HR;Olson,PN;Gorell,TA
用45只6~8周龄的青春期前比格犬研究了伴随着输卵管上皮退行性变的细胞学变化。未处理组的输卵管上皮由未分化的低立方细胞组成，高度为10.3±2.0μm。低立方细胞在雌二醇(E2)刺激下发生肥大、增殖和细胞分化，形成柱状纤毛细胞和分泌细胞。E2处理12天后，上皮细胞完全分化，高度为29.4±2.6μm，56%的细胞具有纤毛。当E2处理再持续12天时，上皮细胞保持分化状态。然而，如果终止E2治疗或单独或与E2联合使用孕酮(P)，输卵管上皮细胞退化，6天后由高度从9到14μm的低立方细胞组成，大约25%的细胞具有纤毛。多种细胞学变化是退化过程的特征。最直接的退化迹象是上皮高度的降低和细胞的存在，这些细胞具有缩小的、多形的核，缺乏显著的核仁。退行性改变包括：(1)上皮细胞顶端细胞质的夹断和脱落；(2)整个细胞和/或细胞核的脱出；(3)纤毛和基底体的吸收。在E2停用或P治疗后的前6天，巨噬细胞和细胞碎片经常出现在输卵管腔内。退化的过程并不是在整个输卵管壶腹部同步进行的，也不是所有的细胞都以同样的方式退化。P治疗后伴随输卵管退行性变的细胞学改变与E2停药后观察到的相同。实验结果表明：(1)E2诱导输卵管上皮细胞分化并维持分化状态；(2)E2停用或P处理导致输卵管上皮细胞退化；(3)柱状纤毛细胞和分泌细胞向低立方细胞的转变至少涉及3个不同的退化过程；(4)整个输卵管壶腹区不同步发生退化。
A total of 45 prepubertal beagles 6 to 8 weeks of age were used to study the cytological changes that accompany regression of the oviductal epithelium. The oviductal epithelium in untreated pups consisted of undifferentiated low cuboidal cells that measured 10.3 ± 2.0 μm in height. In response to estradiol (E2), low cuboidal cells underwent hypertrophy, hyperplasia, and cytodifferentiation and gave rise to columnar ciliated and secretory cells. After 12 days of E2treatment the epithelium was fully differentiated and measured 29.4 ± 2.6 μm in height with 56% of the cells possessing cilia. When E2treatment was continued for an additional 12 days, the epithelium was maintained in a differentiated state. However, if E2treatment was terminated or progesterone (P) given alone or in conjunction with E2, the oviductal epithelium regressed and after 6 days was composed of low cuboidal cells that ranged in height from 9 to 14 μm with approximately 25% of the cells possessing cilia. A variety of cytological changes characterized the process of regression. The most immediate signs that regression was underway were a reduction in the height of the epithelium and the presence of cells with shrunken, pleomorphic nuclei that lacked prominent nucleoli. Degenerative events included: (1) pinching off and shedding of the apical cytoplasm of cells comprising the epithelium, (2) extrusion of whole cells and/or nuclei, and (3) resorption of cilia and basal bodies. During the first 6 days following E2withdrawal or P treatment, macrophages and cellular debris were frequently present within the lumen of the oviduct. The process of regression did not proceed synchronously throughout the ampulla of the oviduct, nor did all cells appear to degenerate in the same manner. The cytological changes that accompanied oviductal regression following P treatment were identical to those observed following E2withdrawal. Results from experiments conducted in the present study show that: (1) E2induces the oviductal epithelium to differentiate and is required to maintain the epithelium in a differentiated state, (2) E2withdrawal or P treatment causes the oviductal epithelium to regress, (3) at least three distinct degenerative processes are involved in the transition of columnar ciliated and secretory cells into low cuboidal cells, and (4) regression does not occur synchronously throughout the ampulla region of the oviduct.