A mouse model for cytomegalovirus infection.
A mouse model for cytomegalovirus infection.
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DOI：
10.1002/0471142735.im1907s43
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发表时间：
2001-08-01
影响因子：
--
通讯作者：
Koszinowski, U H
中科院分区：
文献类型：
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作者：
Brune, W;Hengel, H;Koszinowski, U H
本单元描述了用鼠巨细胞病毒（mCMV）感染新生和成年小鼠的程序。包括在细胞培养物中繁殖mCMV和从感染小鼠的唾液腺制备更具毒性形式的mCMV的方法。提供了一种空斑形成细胞（PFC）试验，用于测量感染组织或病毒原液的mCMV滴度。此外，还描述了一种制备用于繁殖mCMV和PFC试验的鼠胚胎成纤维细胞的方法。
This unit describes procedures for infecting newborn and adult mice with murine cytomegalovirus (mCMV). Methods are included for propagating mCMV in cell cultures and for preparing a more virulent form of mCMV from salivary glands of infected mice. A plaque-forming cell (PFC) assay is provided for measuring mCMV titers of infected tissues or virus stocks. In addition, a method is described for preparing the murine embryonic fibroblasts used for propagating mCMV and for the PFC assay.