A Bartonella vinsonii berkhoffii typing scheme based upon 16S-23S ITS and Pap31 sequences from dog, coyote, gray fox, and human isolates
A Bartonella vinsonii berkhoffii typing scheme based upon 16S-23S ITS and Pap31 sequences from dog, coyote, gray fox, and human isolates
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DOI：
10.1016/j.mcp.2005.11.002
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发表时间：
2006-04-01
影响因子：
3.3
通讯作者：
Breitschwerdt, EB
中科院分区：
文献类型：
--
作者：
Maggi, RG;Chomel, B;Breitschwerdt, EB
自1993年从患有心内膜炎的狗身上分离出伯克菲巴尔通体亚种以来，该生物已成为犬类和人类的重要病原体。目前的证据表明，土狼。狗和灰狐是潜在的宿主。基于16S-23S ITS区和Pap31基因的序列差异，我们提出了一种将文氏贝氏菌亚种划分为文氏贝氏菌亚种的分类方案。Berkhoffii分离株分为四种不同的类型。两个保守序列，分别为37 bp和18 bp。在四种vinsonii亚种的ITS区域内存在差异。berkhoffii类型。迄今为止，在美国已鉴定出文氏伯克氏杆菌I型、II型和III型。第三型在欧洲，第四型在加拿大。基于所提出的基因分型方案，在未来涉及土狼、狗、灰狐、人类和潜在的其他动物或节肢动物媒介巴尔通体感染的分子流行病学研究中，需要更彻底地描述巴尔通体类型的地理分布。菌株分型有助于更好地确定储层潜力、携带模式。不同文氏白僵菌类型的传播方式和地理分布。(c) 2005 Elsevier Ltd版权所有。
Since the isolation of Bartonella vinsonii subspecies berkhoffii from a dog with endocarditis in 1993, this organism has emerged as an important pathogen in dogs and as an emerging pathogen in people. Current evidence indicates that coyotes. dogs and gray foxes potentially serve as reservoir hosts. Based upon sequence differences within the 16S-23S ITS region and Pap31 gene, we propose a classification scheme that divides B. vinsonii subsp. berkhoffii isolates into four distinct types. Two conserved sequences, of 37 and 18 bp, respectively. are differentially present within the ITS region of each of the four B. vinsonii subsp. berkhoffii types. To date, B. vinsonii berkhoffii types I, II, and III have been identified in the US. type III in Europe and type IV in Canada. Based upon the proposed genotyping scheme, the geographic distribution of B. vinsonii berkhoffii types needs to be more thoroughly delineated in future molecular epidemiological studies involving Bartonella infection in coyotes, dogs, gray foxes, human beings and potentially other animals or in arthropod vectors. Strain typing may help to better define the reservoir potential, carriership patterns. modes of transmission, and geographic distribution for each B. vinsonii berkhoffii type. (c) 2005 Elsevier Ltd. All rights reserved.