Assignment of the homologue of the yeast origin recognition complex subunit ORC4 (ORC4L) to human chromosome band 2q22-->q23 by in situ hybridization and somatic cell hybrid analysis.
Assignment of the homologue of the yeast origin recognition complex subunit ORC4 (ORC4L) to human chromosome band 2q22-->q23 by in situ hybridization and somatic cell hybrid analysis.
复制标题
通过原位杂交和体细胞杂交分析，将酵母起源识别复合体亚基 ORC4 (ORC4L) 的同源物分配给人类染色体带 2q22-->q23。
DOI：
10.1159/000014997
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Hanaoka,F
中科院分区：
文献类型：
--
作者：
Eki,T;Dean,FB;Kohda,A;Okumura,K;Abe,M;Murakami,Y;Ishiai,M;Satomoto,K;Hurwitz,J;O'Donnell,M;Hanaoka,F
Initiation of DNA replication in eukaryotic cells is tightly controlled and coordinated with other events during the cell cycle and cell proliferation. The origin recognition complex (ORC) of Saccharomyces cerevisiae consists of six polypeptides (Orc1p to Orc6p)(Loo et al., 1995). In the presence of ATP, this complex binds to several well defined autonomously replicating sequences (ARSs) that serve as chromosomal replication origins. ORC is bound to ARS throughout the cell cycle and could act as a platform for recruiting other proteins involved in the initiation of replication in yeast cells. The genes for all six ORC subunits have been isolated and deletion of any one of these genes results in lethality (Loo et al., 1995). Several cDNA clones encoding proteins homologous to the S. cerevisiae ORC have been isolated from Schizosaccharomyces pombe, Xenopus laevis, Drosophila melanogaster, and humans for both Orc1p and Orc2p, from Kluyveromyces lactis for Orc1p, and from Caenorhabditis elegans, Arabidopsis thaliana, and mouse for Orc2p (GenBank release 103.0). ORC5 homologues have been recently identified in S. pombe, C. elegans, D. melanogaster, and humans (Ishiai et al., 1997). These results indicate that the architecture of the ORC complex is conserved among eukaryotes and suggests that there may be a common mechanism for the initiation of DNA replication in eukaryotic cells (Gavin et al., 1995). Previously we assigned the genes of three other human ORC subunits, ORC1L, ORC2L, and ORC5L, to chromosome bands 1p32, 2q33, and 7q22, respectively (Ishiai et al., 1997; Eki et al., 1996). Here, we have newly assigned the human ORC4L to chromosome 2 band q22→ q23. Because the human ORC is likely to be involved in regulating the initiation of DNA replication and gene transcription, mutations in the Orc4 genes might result in deregulation of these processes. Such aberrations could cause various genetic disorders, including cancers. Partial deletions of 2q22→ q24 were observed in non-Hodgkin’s lymphoma (Berger et al., 1991) and t (2; 14)(q23; q32. 3) was reported as the sole abnormality in a patient with acute nonlymphocytic leukemia (FAB-M4)(Columbano-Green et al., 1990). However, the relationship between these cancers and dysfunctions of the human ORC4 subunit remains to be determined.
