Expression Profile of the Matricellular Protein Osteopontin in Primary Open-Angle Glaucoma and the Normal Human Eye
Expression Profile of the Matricellular Protein Osteopontin in Primary Open-Angle Glaucoma and the Normal Human Eye
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DOI：
10.1167/iovs.11-7409
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发表时间：
2011-08-01
影响因子：
4.4
通讯作者：
Fautsch, Michael P.
中科院分区：
文献类型：
--
作者：
Chowdhury, Uttio Roy;Jea, Seung-Youn;Fautsch, Michael P.
目的.探讨骨桥蛋白（OPN）在原发性开角型青光眼（POAG）及正常眼中的作用。通过酶联免疫吸附测定法在从人供体眼睛（POAG和正常）和手术样品（POAG和选择性白内障摘除术）获得的房水（AH）中进行OPN定量。采用免疫印迹和免疫组织化学方法研究眼组织切片和原代正常人小梁网（NTM）细胞中OPN的表达和定位。分析拉坦前列素游离酸（LFA）处理的NTM细胞的OPN基因和蛋白表达。通过眼压计测量眼压，通过光学相干断层扫描测量年轻OPN-/-和野生型小鼠的中央角膜厚度。与正常AH相比，供体POAG AH中的OPN水平显著降低（0.54 +/- 0.18 ng/μ g [n = 8] vs. 0.77 +/- 0.23 ng/μ g [n = 9]; P = 0.039）。在手术AH中观察到类似的趋势（1.05 +/- 0.31 ng/μ g [n = 20] vs. 1.43 +/- 0.88 ng/μ g [n = 20]; P = 0.083）。OPN存在于小梁网、角膜上皮和内皮、虹膜、睫状体、视网膜、玻璃体液和视神经中。LFA增加OPN基因表达，但观察到OPN蛋白表达的微小变化。在OPN-/-和野生型小鼠之间，眼内压没有差异（17.5 +/- 2.0 mm Hg [n = 56] vs. 17.3 +/- 1.9 mm Hg [n = 68]），但中央角膜厚度较薄（91.7 +/- 3.6 μ m [n = 50] vs. 99.2 +/- 5.5 μ m [n = 70]）。OPN广泛分布于人眼中，在POAG AH中发现浓度较低。年轻小鼠中OPN的减少不影响IOP。（Invest Ophthalmol维斯科学。2011; 52：6443-6451）DOI：10.1167/iovs.11-7409
PURPOSE. To characterize the role of osteopontin (OPN) in primary open-angle glaucoma (POAG) and normal eyes.METHODS. OPN quantification was performed by enzyme-linked immunosorbent assay in aqueous humor (AH) obtained from human donor eyes (POAG and normal) and surgical samples (POAG and elective cataract removal). OPN expression and localization in whole eye tissue sections and primary normal human trabecular meshwork (NTM) cells were studied by Western blot and immunohistochemistry. Latanoprost-free acid (LFA)-treated NTM cells were analyzed for OPN gene and protein expression. Intraocular pressure was measured by tonometry, and central corneal thickness was measured by optical coherence tomography in young OPN-/- and wild-type mice.RESULTS. OPN levels were significantly reduced in donor POAG AH compared with normal AH (0.54 +/- 0.18 ng/mu g [n = 8] vs. 0.77 +/- 0.23 ng/mu g [n = 9]; P = 0.039). A similar trend was observed in surgical AH (1.05 +/- 0.31 ng/mu g [n = 20] vs. 1.43 +/- 0.88 ng/mu g [n = 20]; P = 0.083). OPN was present in the trabecular meshwork, corneal epithelium and endothelium, iris, ciliary body, retina, vitreous humor, and optic nerve. LFA increased OPN gene expression, but minimal change in OPN protein expression was observed. No difference in intraocular pressure (17.5 +/- 2.0 mm Hg [n = 56] vs. 17.3 +/- 1.9 mm Hg [n = 68]) but thinner central corneal thickness (91.7 +/- 3.6 mu m [n = 50] vs. 99.2 +/- 5.5 mu m [n = 70]) was noted between OPN-/- and wild-type mice.CONCLUSIONS. OPN is widely distributed in the human eye and was found in lower concentrations in POAG AH. Reduction of OPN in young mice does not affect IOP. (Invest Ophthalmol Vis Sci. 2011; 52: 6443-6451) DOI: 10.1167/iovs.11-7409