Aberrant expression and localization of hnRNP-A2/B1 is a common event in human gastric adenocarcinoma
Aberrant expression and localization of hnRNP-A2/B1 is a common event in human gastric adenocarcinoma
复制标题
DOI：
10.1111/j.1440-1746.2010.06482.x
复制
发表时间：
2011-01-01
影响因子：
4.1
通讯作者：
Kong, Hai-Yan
中科院分区：
文献类型：
--
作者：
Jing, Guang-Jun;Xu, Dong-Hui;Kong, Hai-Yan
背景和目的：核基质蛋白可以作为癌症病变的蛋白质组标记。本研究旨在探讨异质核核糖核蛋白-A2和B1(hnRNP-A2/B1)在人胃癌发生中的作用。方法：收集人胃癌组织和非癌组织进行免疫组化分析。采用蛋白质组学技术、Western blot、激光共聚焦显微镜、实时定量逆转录-聚合酶链式反应检测核基质蛋白的异常表达。结果：hnRNP-A2/B1存在于胃癌细胞核基质中，在人胃癌中其表达增强，而六亚甲基双乙酰胺则降低其表达。 hnRNP-A2/B1与c-myc、c-fos、p53和Rb的共定位在肿瘤细胞分化过程中从核仁转位到细胞质。结论：hnRNP-A2/B1通过与癌基因和抑癌基因的相互作用影响肿瘤细胞的分化，并且在人胃癌中过表达。我们假设 hnRNP-A2/B1 可以作为诊断人类胃癌的生物标志物。
Background and Aim:Nuclear-matrix proteins can be proteomic markers for cancer lesions. The present study aimed to determine the roles of heterogeneous nuclear ribonucleoproteins-A2 and B1 (hnRNP-A2/B1) in human gastric carcinogenesis.Methods:Human gastric cancer and non-cancerous tissues were collected for immunohistochemical analysis. Proteomics technique, Western blot, laser confocal microscope, and real-time quantitative reverse transcription-polymerase chain reaction were performed to determine the aberrant expression of nuclear-matrix proteins.Results:hnRNP-A2/B1 existed in the nuclear matrix of gastric cancer cells, and its expression was enhanced in human gastric cancer and decreased by hexamethylene bisacetamide. The colocalization of hnRNP-A2/B1 with c-myc, c-fos, p53, and Rb was translocated from the nucleolus to the cytoplasm during the differentiation of tumor cells.Conclusions:hnRNP-A2/B1 affected tumor cell differentiation through interaction with oncogenes and tumor-suppressor genes, and it was overexpressed in human gastric cancer. We postulate that hnRNP-A2/B1 could serve as a biomarker for the diagnosis of human gastric cancer.