Decreased expression of Ras-GRF1 in the brain tissue of the intractable epilepsy patients and experimental rats
Decreased expression of Ras-GRF1 in the brain tissue of the intractable epilepsy patients and experimental rats
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顽固性癫痫患者及实验大鼠脑组织Ras-GRF1表达降低
DOI：
10.1016/j.brainres.2012.11.033
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发表时间：
2013-02
期刊：
影响因子：
--
通讯作者：
Zhu Q, Wang L, Xiao Z, Xiao F, Luo J, Zhang X, Pe
中科院分区：
文献类型：
--
作者：
Zhu Q, Wang L, Xiao Z, Xiao F, Luo J, Zhang X, Pe
Ras-鸟嘌呤核苷酸释放因子1（Ras-GRF 1）主要存在于突触体，其表达在出生后随着神经元回路的发育而增加。有证据表明，Ras-GRF 1可以介导突触可塑性的形式，并可能通过多种信号转导途径参与神经元兴奋性和轴突生长的调节。本研究采用免疫荧光、免疫组化和Western blotting方法检测Ras-GRF 1在药物难治性颞叶癫痫（TLE）患者和氯化锂-匹罗卡品点燃大鼠脑组织中的表达，探讨Ras-GRF 1在TLE中的可能作用。我们随机选择了30例颞叶肿瘤患者的颞叶新皮质组织和9例正常对照组的颞叶新皮质组织。同时，观察癫痫大鼠和对照大鼠不同时相（急性期、潜伏期和慢性期）Ras-GRF 1蛋白表达的分布和水平。Ras-GRF 1主要表达于神经元的质膜和树突，但在癫痫患者和癫痫大鼠脑组织中不与GFAP阳性星形胶质细胞共表达。与对照组相比，TLE患者的Ras-GRF 1表达显著降低。Ras-GRF 1在癫痫大鼠中的表达在癫痫发作后1天已经降低，然后在潜伏期逐渐降低，并在慢性期达到最低水平。提示Ras-GRF 1表达降低可能参与了TLE的发病过程。
Ras-guanine nucleotide-releasing factor 1 (Ras-GRF1) is present mainly at synaptosome and its expression after birth increases in parallel with the development of neuronal circuitry. Evidences suggested that Ras-GRF1 could mediate forms of synaptic plasticity and might participate in the regulation of neuronal excitability and neurite outgrowth though various signal transduction pathway. The aim of this study was to measure Ras-GRF1 expression in brain tissue of patients with drug-refractory temporal lobe epilepsy (TLE) and lithium chloride-pilocarprine kindled rats using double-label immunofluorescence, immunohistochemistry and Western blotting and to discuss the possible role of Ras-GRF1 in TLE. We randomly selected 30 temporal neocortices tissues from patients with TLE and 9 histologically normal temporal neocortices samples from controls. Meanwhile, we investigated the distribution and level of Ras-GRF1 protein expression during the different phases (the acute period, the latent period and the chronic phase) in the epileptic and control rats. Ras-GRF1 was mainly expressed in the plasma membrane and dendrite of neurons, but it was not co-expressed with GFAP-positive astrocytes in the brain tissue of patients and epileptic rats. Compared with controls, Ras-GRF1 expression was significantly decreased in TLE patients. Ras-GRF1 expression in epileptic rats was already reduced at 1 day post-seizures, then gradually decreased during the latent period and reached a minimum level during the chronic phase. These results demonstrated that the decreased expression of Ras-GRF1 could be involved in the pathogenesis of human TLE.
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DOI：
--
发表时间：
1994-04
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
K. Touhara;James Inglese;J. Pitcher;G. Shaw;R. Lefkowitz
通讯作者：
K. Touhara;James Inglese;J. Pitcher;G. Shaw;R. Lefkowitz
影响因子：
3.5
作者：
Darcy, Michael J.;Trouche, Stephanie;Jin, Shan-Xue;Feig, Larry A.
通讯作者：
Feig, Larry A.
DOI：
10.1523/jneurosci.20-13-05024.2000
发表时间：
2000-07
期刊：
The Journal of Neuroscience
影响因子：
--
作者：
Wai T. Wong;B. Faulkner-Jones;J. Sanes;R. Wong
通讯作者：
Wai T. Wong;B. Faulkner-Jones;J. Sanes;R. Wong
影响因子：
3.5
作者：
Jae-Won Yang;Thomas Czech;M. Felizardo;C. Baumgartner;Gert Lubec
通讯作者：
Jae-Won Yang;Thomas Czech;M. Felizardo;C. Baumgartner;Gert Lubec
DOI：
--
发表时间：
1992
期刊：
Epilepsy research. Supplement
影响因子：
--
作者：
Luiz E. Mello;E. Cavalheiro;Tan Am;J. Pretorius;T. Babb;D. Finch
通讯作者：
Luiz E. Mello;E. Cavalheiro;Tan Am;J. Pretorius;T. Babb;D. Finch