Contacts between the commissural axons and the floor plate cells are mediated by nectins
Contacts between the commissural axons and the floor plate cells are mediated by nectins
复制标题
DOI：
10.1016/j.ydbio.2004.05.034
复制
发表时间：
2004-09-15
影响因子：
2.7
通讯作者：
Takai, Y
中科院分区：
文献类型：
--
作者：
Okabe, N;Shimizu, K;Takai, Y
在中枢神经系统(CNS)发育过程中，连合轴突向腹中线生长。穿过底板后，它们的轨迹突然从圆周向纵轴改变。这种轴突引导涉及连合轴突和底板细胞之间的接触，但其机制或结构尚不完全清楚。在本研究中，我们发现免疫球蛋白样细胞-细胞黏附分子Nectin-1和-3分别不对称地定位于连合轴突和底板细胞之间的接触点。在体外，干扰Nectin-1和-3之间的内源性反式相互作用导致大鼠后脑连合轴突的异常颤动和接触损伤，并导致大鼠对侧后脑连合轴突的纵向旋转失败。这些结果表明，连合轴突与底板细胞之间的接触是由Nectin-1和-3之间的异反式相互作用介导的，并参与了连合轴突轨迹的调节。(C)2004 Elsevier Inc.保留所有权利。
During development of the central nervous system (CNS), commissural axons grow toward the ventral midline. After crossing the floor plate, they abruptly change their trajectory from the circumferential to the longitudinal axis. The contacts between the commissural axons and the floor plate cells are involved in this axonal guidance, but their mechanisms or structures have not fully been understood. In this study, we found that nectin-1 and -3, immunoglobulin-like cell-cell adhesion molecules, asymmetrically localized at the contact sites between the commissural axons and the floor plate cells, respectively. In vitro perturbation of the endogenous trans-interaction between nectin-1 and -3 caused abnormal fasciculation of the commissural axons and impairment of the contacts, and resulted in failure in longitudinal turns of the commissural axons at the contralateral sites of the rat hindbrain. These results indicate that the contacts between the commissural axons and the floor plate cells are mediated by the hetero-trans-interaction between nectin-1 and -3 and involved in regulation of the trajectory of the commissural axons. (C) 2004 Elsevier Inc. All rights reserved.