The "one airway, one disease" concept in light of Th2 inflammation
The "one airway, one disease" concept in light of Th2 inflammation
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DOI：
10.1183/13993003.00437-2018
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发表时间：
2018-10-01
影响因子：
24.3
通讯作者：
Barbry, Pascal
中科院分区：
文献类型：
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作者：
Giovannini-Chami, Lisa;Paquet, Agnes;Barbry, Pascal
根据变应性呼吸道疾病中鼻和支气管之间的病理生理连续体描述，我们评估鼻上皮是否可以反映支气管上皮的2型辅助性T细胞（Th2）状态。（AR，n=12）和患有孤立性变应性鼻炎的患者（R，n=14）。通过流式细胞术评估细胞组成，通过RNA测序分析基因表达，并从文献中获得Th2、17型辅助性T细胞（Th17）和干扰素（IFN）特征。来自AR组的所有支气管样品都是Th2高的。在71%的病例中，AR组鼻样本的基因表达谱正确预测了配对支气管样本的Th2状态。然而，鼻细胞似乎不是Th2应答的可靠替代物，特别是由于在26个鼻样品中的14个中IFN应答的更强的影响。鼻和支气管中的Th2评分与肥大细胞计数相关（均为p
In line with the pathophysiological continuum described between nose and bronchus in allergic respiratory diseases, we assessed whether nasal epithelium could mirror the Type 2 T-helper cell (Th2) status of bronchial epithelium.Nasal and bronchial cells were collected by brushing from healthy controls (C, n=13), patients with allergic rhinitis and asthma (AR, n=12), and patients with isolated allergic rhinitis (R, n=14). Cellular composition was assessed by flow cytometry, gene expression was analysed by RNA sequencing and Th2, Type 17 T-helper cell (Th17) and interferon (IFN) signatures were derived from the literature.Infiltration by polymorphonuclear neutrophils (PMN) in the nose excluded 30% of the initial cohort. All bronchial samples from the AR group were Th2-high. The gene expression profile of nasal samples from the AR group correctly predicted the paired bronchial sample Th2 status in 71% of cases. Nevertheless, nasal cells did not appear to be a reliable surrogate for the Th2 response, in particular due to a more robust influence of the IFN response in 14 out of 26 nasal samples. The Th2 scores in the nose and bronchi correlated with mast cell count (both p