Caenimonas sedimenti sp. nov., Isolated from Sediment of the Wastewater Outlet of an Agricultural Chemical Plant
Caenimonas sedimenti sp. nov., Isolated from Sediment of the Wastewater Outlet of an Agricultural Chemical Plant
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DOI：
10.1007/s00284-020-02145-6
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发表时间：
2020-08
影响因子：
2.6
通讯作者：
Jianyi Xu;Mengyao Sheng;Zhou Yang;J. Qiu;Jun Zhang;Lei Zhang;Jian He
中科院分区：
文献类型：
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作者：
Jianyi Xu;Mengyao Sheng;Zhou Yang;J. Qiu;Jun Zhang;Lei Zhang;Jian He
A novel bacterial strain, designated HX-9-20T, was isolated from the sediment collected from the wastewater outlet of an agricultural chemical plant in Maanshan city, Anhui province, PR China. Cells of strain HX-9-20Twere Gram-staining-negative, rod-shaped, translucent, non-motile, and strictly aerobic. Growth was observed between 15 and 35 °C (optimum 30 °C), at pH 6.0–8.0 (optimum pH 7.0), and in the presence of 0–0.4% (w/v) NaCl (optimum 0.2%). The predominant cellular fatty acids were summed feature 3 (C16:1ω7c and/or C16:1ω6c), C16:0and summed feature 8 (C18:1ω6c and/or C18:1ω7c). The major quinone was ubiquinone Q-8. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, and an aminophospholipid. Strain HX-9-20Tcontains 2-hydroxyputrescine and putrescine as the major polyamine. A phylogenetic analysis based on 16S rRNA gene sequences revealed that strain HX-9-20Twas affiliated with the genusCaenimonas, exhibiting the highest sequence similarities withCaenimonas koreensisEMB320T(97.3% similarity) andRamlibacter humi18×22-1T(97.0%), and less than 97.0% similarity with other type strains. The average nucleotide identity (ANI) and digital DNA–DNA hybridization values (dDDH) between HX-9-20TandC. koreensisEMB320Twere 76.9% and 23.5% respectively. The ANI and dDDH between HX-9-20TandR. humi18×22-1Twere 80.3% and 23.6%, respectively. The G + C content of the genomic DNA was 67.5 mol%. On the basis of the polyphasic taxonomic data, strain HX-9-20Trepresents a novel species of the genusCaenimonas, for which the nameCaenimonas sedimentisp. nov. is proposed. The type strain is HX-9-20T(=KCTC 72473T=CCTCC AB 2019266T).
