Quantification of N-glycosylation site occupancy status based on labeling/label-free strategies with LC-MS/MS.
Quantification of N-glycosylation site occupancy status based on labeling/label-free strategies with LC-MS/MS.
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DOI：
10.1016/j.talanta.2017.04.053
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发表时间：
2017-08
期刊：
影响因子：
6.1
通讯作者：
Shu Zhang;Wei Li;Haojie Lu;Yin‐kun Liu
中科院分区：
文献类型：
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作者：
Shu Zhang;Wei Li;Haojie Lu;Yin‐kun Liu
蛋白质N-糖基化在生理和病理过程中起着重要作用。描述位点特异性N-糖基化，包括N-聚糖的宏观异质性（糖基化位点占有率）和微观异质性（位点特异性聚糖结构）对于理解糖蛋白的生物合成和功能是重要的。N-糖链的宏观异质性是糖蛋白的一种生理特性，其调控机制和功能的研究一直受到技术上的限制。N-糖基化位点占有率的定量将揭示宏观异质性在各种生物学特性中的关键作用。基于液相色谱（LC）-质谱（MS）的定量正在成为糖基化表征的有力工具。本文综述了近年来用于定量N-糖基化位点占有率的标记和无标记定量质谱方法，包括靶糖蛋白的定量方法。
Protein N-glycosylation plays important roles in physiological and pathological processes. Characterizing the site-specific N-glycosylation including N-glycan macroheterogeneity (glycosylation site occupancy) and microheterogeneity (site-specific glycan structure) is important for understanding of glycoprotein biosynthesis and function. N-Glycan macroheterogeneity is a physiological property of glycoprotein and the technical obstacles have restricted research into the regulation and functions of this heterogeneity. Quantification of N-glycosylation site occupancy would uncover the critical role of macroheterogeneity in a variety of biological properties. Liquid chromatography (LC)- mass spectrometry (MS)-based quantification is emerging as a powerful tool for glycosylation characterization. This review summarizes the labeling and label-free quantitative MS approaches for quantifying N-glycosylation site occupancy, including its quantification for target glycoproteins in recent years.