Proteome changes in the plasma of Papilio xuthus (Lepidoptera: Papilionidae): effect of parasitization by the endoparasitic wasp Pteromalus puparum (Hymenoptera: Pteromalidae)
Proteome changes in the plasma of Papilio xuthus (Lepidoptera: Papilionidae): effect of parasitization by the endoparasitic wasp Pteromalus puparum (Hymenoptera: Pteromalidae)
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DOI：
10.1631/jzus.b0820314
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发表时间：
2009-05
期刊：
影响因子：
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通讯作者：
Jiaying Zhu;G. Yè;Qi Fang;Cui Hu
中科院分区：
文献类型：
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作者：
Jiaying Zhu;G. Yè;Qi Fang;Cui Hu
尽管寄生物与宿主相互作用的生化剖析已得到很好的表征，但有关它们的分子知识却很少。为了了解宿主对寄生蜂攻击的免疫反应的分子基础，我们利用蛋白质组学方法探讨了被内寄生黄蜂 Pteromalus puparum 寄生的 Papilio xuthus 的反应。通过二维 (2D) 电泳检查寄生和未寄生宿主蛹中血浆蛋白的差异表达，发现与未寄生对照样本相比，16 种蛋白与寄生相关。所有这些都通过质谱分析和数据库搜索进行鉴定。发现调节的蛋白质属于以下功能组：体液或细胞免疫、解毒、能量代谢等。这项研究有助于深入了解寄生蜂与其宿主昆虫之间关系的分子机制。
Although the biochemical dissection of parasitoid-host interactions is becoming well characterized, the molecular knowledge concerning them is minimal. In order to understand the molecular bases of the host immune response to parasitoid attack, we explored the response of Papilio xuthus parasitized by the endoparasitic wasp Pteromalus puparum using proteomic approach. By examining the differential expression of plasma proteins in the parasitized and unparasitized host pupae by two-dimensional (2D) electrophoresis, 16 proteins were found to vary in relation to parasitization compared with unparasitized control samples. All of them were submitted to identification by mass spectrometry coupled with a database search. The modulated proteins were found to fall into the following functional groups: humoral or cellular immunity, detoxification, energy metabolism, and others. This study contributes insights into the molecular mechanism of the relationships between parasitoids and their host insects.