DNA polymorphism analysis of candidate genes for type 2 diabetes mellitus in a Mexican ethnic group
DNA polymorphism analysis of candidate genes for type 2 diabetes mellitus in a Mexican ethnic group
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DOI：
10.1016/j.anngen.2004.05.004
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发表时间：
2004-10-01
期刊：
影响因子：
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通讯作者：
Sánchez-Corona, J
中科院分区：
文献类型：
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作者：
Flores-Martínez, SE;Islas-Andrade, S;Sánchez-Corona, J
2型糖尿病是一种复杂的代谢紊乱，由许多遗传和环境因素的作用和相互作用引起。已有研究表明，参与葡萄糖代谢的基因多态性与2型糖尿病的易感性有关。虽然患2型糖尿病的风险随着年龄以及肥胖和高血压的增加而增加，但其流行率和发病率在地理区域和种族群体之间存在差异。在墨西哥，据描述，该国南部的流行率和发病率较高，城市和农村社区之间存在差异。我们研究了来自圣地亚哥·亚米尔特佩克的73个个体，这是墨西哥瓦哈卡州的一个小型土著社区。该人群2型糖尿病患病率较高，本研究的目的是分析Pst I（胰岛素基因）、Nsi I（胰岛素受体基因）和Gly 972 Arg（胰岛素受体底物1基因）多态性与该人群2型糖尿病、肥胖和高血压的关系。临床评价包括BMI和血压测量，生化分析包括空腹血浆胰岛素和葡萄糖水平的测定。应用PCR和限制性内切酶分析技术对基因组DNA进行多态性分析。从这里进行的统计分析，单独地，Pst 1，Nsi 1和Gly972Arg多态性与2型糖尿病，肥胖或高血压表型在这个人群中不相关。然而，Nsi 1和Pst 1多态性与血清胰岛素水平升高之间存在关联。(C)2004年，Elsevier SAS。All rights reserved.
Type 2 diabetes mellitus is a complex metabolic disorder resulting from the action and interaction of many genetic and environmental factors. It has been reported that polymorphisms in genes involved in the metabolism of glucose are associated with the susceptibility to develop type 2 diabetes mellitus. Although the risk of developing type 2 diabetes mellitus increases with age, as well as with obesity and hypertension, its prevalence and incidence are different among geographical regions and ethnic groups. In Mexico, a higher prevalence and incidence has been described in the south of the country, and differences between urban and rural communities have been observed. We studied 73 individuals from Santiago Jamiltepec, a small indigenous community from Oaxaca State, Mexico. This population has shown a high prevalence of type 2 diabetes mellitus, and the aim of this study was to analyze the relationship between the Pst I (insulin gene), Nsi I (insulin receptor gene) and Gly972Arg (insulin receptor substrate 1 gene) polymorphisms and type 2 diabetes mellitus, obesity and hypertension in this population. Clinical evaluation consisted of BMI and blood pressure measurements, and biochemical assays consisted of determination of fasting plasma insulin and glucose levels. PCR and restriction enzyme digestion analysis were applied to genomic DNA to identify the three polymorphisms. From statistical analysis carried out here, individually, the Pst 1, Nsi 1 and Gly972Arg polymorphisms were not associated with the type 2 diabetes, obese or hypertensive phenotypes in this population. Nevertheless, there was an association between the Nsi 1 and Pst 1 polymorphisms and increased serum insulin levels. (C) 2004 Elsevier SAS. All rights reserved.