Common and idiosyncratic patterns of cytokine gene expression by Epstein-Barr virus transformed human B cell lines
Common and idiosyncratic patterns of cytokine gene expression by Epstein-Barr virus transformed human B cell lines
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DOI：
10.1089/vim.1997.10.183
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发表时间：
1997-01-01
期刊：
影响因子：
2.2
通讯作者：
Hobbs, MV
中科院分区：
文献类型：
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作者：
Rochford, R;Cannon, MJ;Hobbs, MV
EB病毒（EBV）转化的人B细胞在体外无限增殖，并且已经提出，精氨酸介导的自分泌环有助于维持淋巴母细胞样表型。我们使用了一种新的多探针RNA酶保护试验来定量EBV转化的淋巴母细胞样细胞系（LCL）表达的细胞因子mRNA种类，这些细胞系通过用B 95 -8或野生型EBV转化B细胞或通过EBV相关B细胞淋巴瘤的体外生长来获得，以鉴定在所有LCL中通常表达的细胞因子，从而更可能是B细胞永生化所必需的。所有16例LCL均表达高水平的肿瘤坏死因子（TNF）α、TNF β和转化生长因子（TGF）PI mRNA，而大多数LCL中检测到白细胞介素（IL）-10转录物，但水平较低。IL-1 α、IL-1 β、IL-6、IL-12 p35、IL-12 p40、IL-13和IFN γ mRNA的表达在所测试的LCL中是可变的。粒细胞-巨噬细胞集落刺激因子（GM-CSF）、IL-2、IL-4和IL-5 mRNA在所有LCL中均未检测到。此外，我们发现，IL-10，TNF α，和TNF β mRNA诱导EBV阴性B细胞系感染后，与EBV。这些数据定义了LCL的细胞因子表达的常见模式与特异质模式，在前一种情况下，诸如TNF α、TNF β和IL-10的细胞因子作为EBV永生化B细胞的自分泌调节所必需的强有力的候选者出现。
Epstein-Barr virus (EBV) transformed human B cells proliferate indefinitely in vitro, and it has been proposed that cytokine-mediated autocrine loops contribute to the maintenance of the lymphoblastoid phenotype. We used a novel multiprobe RNase protection assay to quantify cytokine mRNA species expressed by EBV-transformed lymphoblastoid cell lines (LCL), derived either by the transformation of B cells with B95-8 or wild-type EBV or by the in vitro outgrowth of EBV-associated B cell lymphomas to identify cytokines that are commonly expressed in all LCL and thus more likely to be essential for immortalization of B cells. All 16 LCL expressed high levels of tumor necrosis factor (TNF)alpha, TNF beta, and transforming growth factor (TGF)PI mRNA, while interleukin (IL)-10 transcripts were detected in most LCL but at a lower level. Expression of IL-1 alpha; IL-1 beta, IL-6, IL-12p35, IL-12p40, IL-13 and IFN gamma mRNA was variable among the LCL tested. Granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-2, IL-4, and IL-5 mRNA were undetectable in all LCL. Furthermore, we found that IL-10, TNF alpha, and TNF beta mRNA were induced in EBV-negative B cell lines after infection with EBV. These data define common versus idiosyncratic patterns of cytokine expression by LCL and, in the former case, such cytokines as TNF alpha, TNF beta, and IL-IO emerge as strong candidates that are essential for the autocrine regulation of EBV-immortalized B cells.