Gamma-Aminobutyric Acid (GABA) Attenuates Ischemia Reperfusion-Induced Alterations in Intestinal Immunity
Gamma-Aminobutyric Acid (GABA) Attenuates Ischemia Reperfusion-Induced Alterations in Intestinal Immunity
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DOI：
10.1248/bpb.b18-00338
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发表时间：
2018-12-01
影响因子：
2
通讯作者：
Iseki, Ken
中科院分区：
文献类型：
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作者：
Kubota, Atsuhito;Kobayashi, Masaki;Iseki, Ken
本研究的目的是确定γ-氨基丁酸（GABA）对免疫球蛋白A（伊加）从派尔集合淋巴结（PP）细胞分泌的影响，以评估大鼠α-防御素-5（RD-5）在大鼠小肠中的表达，并确定GABA对肠缺血再灌注（I/R）损伤诱导的肠道先天免疫的影响。我们发现，GABA引起的伊加分泌增加的存在和不存在的脂多糖（LPS）。此外，GABA还显着增加RD-5和超氧化物歧化酶（SOD）1，3的mRNA水平。肠I/R诱导的上级肠系膜动脉闭塞30分钟，然后再灌注60分钟。这导致伊加分泌的显着下降，和RD-5和SOD 1-3的mRNA水平在回肠。另一方面，在I/R诱导前给予GABA对氧化损伤具有显著的保护作用，并减弱了对肠道免疫的影响。
The aims of this study were to determine the effects of gamma-aminobutyric acid (GABA) on immunoglobulin A (IgA) secretion from Peyer's patch (PP) cells; to assess rat alpha-defensin-5 (RD-5) expression in the rat small intestine; and to determine the effect of GABA on intestinal ischemia reperfusion (I/R) injury-induced intestinal innate immunity. We found that GABA caused an increase in IgA secretion in the presence and absence of lipopolysaccharide (LPS). Moreover, GABA also significantly increased the mRNA levels of RD-5 and superoxide dismutase (Sod) 1, 3. Intestinal I/R was induced by a 30-min occlusion of the superior mesenteric artery followed by a reperfusion for 60-min. This led to a significant decrease in IgA secretion, and mRNA levels of RD-5 and Sod 1-3 in the ileum. On the other hand, administration of GABA before I/R induction had a significant protective effect against oxidative injury and attenuated the effects on intestinal immunity.