Sensory Nerves Affect Bone Regeneration in Rabbit Mandibular Distraction Osteogenesis
Sensory Nerves Affect Bone Regeneration in Rabbit Mandibular Distraction Osteogenesis
复制标题
感觉神经影响兔下颌牵引成骨的骨再生
DOI：
10.7150/ijms.31883
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发表时间：
2019-01-01
影响因子：
3.6
通讯作者：
Wang, Xudong
中科院分区：
文献类型：
--
作者：
Cao, Jian;Zhang, Shijian;Wang, Xudong
目的：探讨下牙槽神经对兔下颌骨牵张成骨过程中新骨形成的影响。方法：20只新西兰白兔以1毫米/天的速度进行双侧牵张成骨。手术显微镜下切除一侧下牙槽神经，下牙槽血管完整。对侧进行假手术。在巩固时间28天时处死兔子。对再生愈伤组织进行X线检查、双能X射线吸收测定、苏木精和伊红染色以及组织形态学分析。使用SPSS 16.0软件包进行配对t检验。结果：下颌去神经侧牵张间隙新生骨的BMD显着低于对照侧（P<0.05）。组织学观察显示，去神经侧骨小梁排列杂乱，软骨细胞分散，而对照侧骨小梁排列整齐，血管丰富，无软骨细胞。去神经侧新生骨体积和新生骨小梁厚度均显着低于对照组（P < 0.05）。结论：下颌骨牵张成骨过程中感觉神经的丧失可能导致新生骨质量下降。
Objectives: To investigate the effects of inferior alveolar nerve on new bone formation in rabbit mandibular distraction osteogenesis. Methods: 20 New Zealand White rabbits underwent bilateral distraction osteogenesis with a rate of 1 mm/day. The inferior alveolar nerve of one side was resected under the surgical microscope, with the inferior alveolar vascular intact. The contralateral side received sham operation. The rabbits were sacrificed at consolidation time of 28 days. The regenerate callus underwent radiograph examination, dual-energy X-ray absorptiometry, haematoxylin and eosin staining and histomorphometric analysis. A paired t-test was performed using SPSS 16.0 software package. Results: The BMD of the new bone in the distraction gap on the denervation side of mandibular was significantly lower (P<0.05) than on the control side. The histological investigation showed that the bone trabeculae were dis-arrayed containing dispersed cartilage cells on the denervation side, whereas the bone trabeculae were orderly with rich blood vessels and no cartilage cell on the control side. Both new bone volume and the thickness of new trabeculae were significantly lower on the denervation side than on the control side (P < 0.05). Conclusion: The loss of the sensory nerves could result in a decrease of the new bone quality during the mandibular distraction osteogenesis.