Human brain synembryn interacts with Gsα and Gqα and is translocated to the plasma membrane in response to isoproterenol and carbachol
Human brain synembryn interacts with Gsα and Gqα and is translocated to the plasma membrane in response to isoproterenol and carbachol
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DOI：
10.1002/jcp.10300
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发表时间：
2003-05-01
影响因子：
5.6
通讯作者：
Olate, J
中科院分区：
文献类型：
--
作者：
Klattenhoff, C;Montecino, M;Olate, J
异源三聚体G蛋白将信号从七螺旋跨膜受体传递到不同的效应系统，调节多种复杂的细胞内途径和功能。在脑中，去极化介导的神经递质释放促进突触传递是由Gsalpha和Gqalpha介导的。为了确定效应的α-蛋白质，我们进行了酵母双杂交筛选的人脑cDNA文库，使用人类气体蛋白作为诱饵。我们确定了一个蛋白质成员的synembryn家庭作为一个相互作用的蛋白质。将研究扩展到其他Galalpha。亚基，我们发现，Gqa也与synembryn相互作用，这些相互作用证实了体外下拉研究和体内共聚焦激光显微镜分析。此外，synembryn易位到质膜卡巴胆碱和异丙肾上腺素的反应。这项研究支持了C.其中，通过遗传研究，共胚蛋白被证明与Gqa一起调节神经递质释放。基于这些观察，我们提出，synembryn在人类神经细胞中发挥着类似的作用。J.细胞。195：151-157，2003。(C)2003 Wiley-Liss，Inc.
Heterotrimeric G-proteins transduce signals from heptahelical transmembrane receptors to different effector systems, regulating diverse complex intracellular pathways and functions. In brain, facilitation of depolarization-incluced neurotransmitter release for synaptic transmission is mediated by Gsalpha and Gqalpha. To identify effectors for Galpha-proteins, we performed a yeast two-hybrid screening of a human brain cDNA library, using the human Gas protein as a bait. We identified a protein member of the synembryn family as one of the interacting proteins. Extending the study to other Galpha. subunits, we found that Gqa also interacts with synembryn, and these interactions were confirmed by in vitro pull down studies and by in vivo confocal laser microscopy analysis. Furthermore, synembryn was shown to translocate to the plasma membrane in response to carbachol and isoproterenol. This study supports recent findings in C. elegans where, through genetic studies, synembryn was shown to act together with Gqa regulating neuronal transmitter release. Based on these observations, we propose that synembryn is playing a similar role in human neuronal cells. J. Cell. Physiol. 195: 151-157, 2003. (C) 2003 Wiley-Liss, Inc.