Flow cytometric analysis of human bone marrow. II. Normal B lymphocyte development.
Flow cytometric analysis of human bone marrow. II. Normal B lymphocyte development.
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DOI：
10.1182/blood.v70.5.1316.bloodjournal7051316
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发表时间：
1987-11
期刊：
影响因子：
20.3
通讯作者：
M. Loken;V. O. Shah;Karen L. Dattilio;C. Civin
中科院分区：
文献类型：
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作者：
M. Loken;V. O. Shah;Karen L. Dattilio;C. Civin
用一组B淋巴细胞反应性单抗分析了正常人骨髓中伴随B淋巴细胞发育的表型变化。采用光散射法和流式细胞仪检测CD19的表达。然后使用定量三色免疫荧光来关联这些细胞上的其他细胞表面抗原，这些细胞被鉴定为正常骨髓中的B淋巴样细胞。CD10和CD20确定了几乎排他的群体，并提供了一种方便的方法来区分不太成熟和更成熟的B系细胞。CD10+细胞可以用CD34进一步细分。CD19+、CD10+、CD34+细胞仅占骨髓细胞的0.6%，但这些细胞含有大部分末端脱氧核苷酸转移酶(TDT+)细胞。在对II类抗原的评估中，在发育过程中，所有B系细胞都表达了HLA-DR，而在发育过程中，HLA-DP的出现先于HLA-DQ。在B系细胞上表达的较晚的抗原中，细胞表面的IgM、CD20和HLA-DQ基本上同时表达。当CD21和CD22在细胞表面获得时，细胞表面的CD10丢失。这些数据表明，多参数流式细胞术可以用来定义基于细胞表面抗原表达的B淋巴细胞发育阶段的连续进展，即使这些细胞只占正常骨髓细胞的一小部分。
A panel of B lymphoid-reactive monoclonal antibodies was used to analyze the phenotypic changes that accompany B lymphocyte development in normal human bone marrow. The B lymphoid cells were identified using light scattering and the expression of CD19 on a flow cytometer. Quantitative three-color immunofluorescence was then used to correlate other cell surface antigens on these cells identified as B lymphoid in normal marrow. CD10 and CD20 identified almost exclusive populations and provided a convenient means of discriminating between the less and more mature B lineage cells. The CD10+ cells could be further subdivided using CD34. The population of CD19+, CD10+, CD34+ cells comprised only 0.6% of marrow cells, but these contained the majority of terminal deoxynucleotidyl transferase (TdT+) cells. In the assessment of class II antigens, HLA-DR was expressed on all B lineage cells whereas HLA-DP preceded HLA-DQ in appearance during the developmental process. Among the later antigens expressed on B lineage cells, cell surface IgM, CD20, and HLA-DQ were expressed at essentially the same time. Cell surface CD10 was lost at the time when CD21 and CD22 were acquired on the cell surface. These data illustrate that multiparameter flow cytometry can be used to define a continuous progression of stages of B lymphocyte development based on cell surface antigen expression even though these cells represent a minor fraction of normal marrow cells.