RasGRP, a Ras guanyl nucleotide-releasing protein with calcium- and diacylglycerol-binding motifs
RasGRP, a Ras guanyl nucleotide-releasing protein with calcium- and diacylglycerol-binding motifs
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DOI：
10.1126/science.280.5366.1082
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发表时间：
1998-05-15
期刊：
影响因子：
56.9
通讯作者：
Stone, JC
中科院分区：
文献类型：
--
作者：
Ebinu, JO;Bottorff, DA;Stone, JC
RasGRP 是一种鸟苷三磷酸酶 Ras 的鸟苷酸释放蛋白，已得到表征。除了催化结构域外，RasGRP 还具有一对非典型的结合钙的“EF 手”和一个二酰基甘油 (DAG) 结合结构域。 RasGRP 激活 Ras 并引起成纤维细胞转化。 DAG 类似物引起 Ras-Erk 信号传导的持续激活和细胞形态的变化。信号传导与 RasGRP 蛋白分配到膜部分相关。当 DAG 结合域被删除时，持续的配体诱导的信号传导和膜分配就不存在。 RasGRP 在神经系统中表达，它可能将 DAG 的变化以及可能的钙浓度的变化与 Ras 激活结合起来。
RasGRP, a guanyl nucleotide-releasing protein for the small guanosine triphosphatase Ras, was characterized. Besides the catalytic domain, RasGRP has an atypical pair of "EF hands" that bind calcium and a diacylglycerol (DAG)-binding domain. RasGRP activated Ras and caused transformation in fibroblasts. A DAG analog caused sustained activation of Ras-Erk signaling and changes in cell morphology. Signaling was associated with partitioning of RasGRP protein into the membrane fraction. Sustained ligand-induced signaling and membrane partitioning were absent when the DAG-binding domain was deleted. RasGRP is expressed in the nervous system, where it may couple changes in DAG and possibly calcium concentrations to Ras activation.