Neuroprotective effects of bilobalide, a component of the Ginkgo biloba extract (EGb 761), in gerbil global brain ischemia
Neuroprotective effects of bilobalide, a component of the Ginkgo biloba extract (EGb 761), in gerbil global brain ischemia
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DOI：
10.1016/s0006-8993(01)03188-2
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发表时间：
2001-12-20
期刊：
影响因子：
2.9
通讯作者：
Fiskum, G
中科院分区：
文献类型：
--
作者：
Chandrasekaran, K;Mehrabian, Z;Fiskum, G
银杏叶提取物（EGb 761）对缺血性损伤的神经保护作用已在动物模型中得到证实。本研究我们比较了白果内酯的保护作用，白果内酯是一种从EGb 761中纯化的萜内酯。和EGb 761抗缺血性损伤。我们测量了沙鼠海马易损区神经元的损失和线粒体DNA（mtDNA）编码的细胞色素氧化酶（考克斯）亚基III mRNA的水平。短暂全前脑缺血5分钟后再灌注7天，海马CAI神经元中神经元死亡显著增加，考克斯III mRNA显著降低。口服EGb 761 25.缺血前连续7天给予50和100 mg/kg/天剂量和3和6 mg/kg/天剂量的白果内酯，可逐渐保护CAI神经元免于死亡和缺血诱导的考克斯III mRNA减少。另外。银杏内酯和EGb 761都能在再灌注第1天，在CAI神经元死亡之前，防止缺血诱导的CAI神经元中考克斯III mRNA的减少。这些结果表明，口服银杏内酯和EGb 761可防止缺血诱导的神经元死亡和线粒体基因表达减少。(C)2001 Elsevier Science B. V.保留所有权利。
The neuroprotective effect of Ginkgo biloba extract (EGb 761) against ischemic injury has been demonstrated in animal models. In this study. we compared the protective effect of bilobalide, a purified terpene lactone from EGb 761. and EGb 761 against ischemic injury. We measured neuronal loss and the levels of mitochondrial DNA (mtDNA)-encoded cytochrome oxidase (COX) subunit III mRNA in vulnerable hippocampal regions of gerbils. At 7 days of reperfusion after 5 min of transient global forebrain ischemia, a significant increase in neuronal death and a significant decrease in COX III mRNA were observed in the hippocampal CAI neurons. Oral administration of EGb 761 at 25. 50 and 100 mg/kg/day and bilobalide at 3 and 6 mg/kg/day for 7 days before ischemia progressively protected CAI neurons from death and from ischemia-induced reductions in COX III mRNA. In addition. both bilobalide and EGb 761 protected against ischemia-induced reductions in COX III mRNA in CAI neurons prior to their death, at I day of reperfusion. These results suggest that oral administration of bilobalide and EGb 761 protect against ischemia-induced neuron death and reductions in mitochondrial gene expression. (C) 2001 Elsevier Science B.V. All rights reserved.