Assessment of viability of human fat injection into nude mice with micro-computed tomography.-猫眼课题宝

DOI：

10.3791/52217

发表时间：

2015-01

期刊：

Journal of visualized experiments : JoVE

影响因子：

通讯作者：

中科院分区：

其他

文献类型：

作者：

David A. Atashroo;Kevin J. Paik;M. Chung;A. McArdle;K. Senarath-Yapa;Elizabeth R. Zielins;R. Tevlin;Christopher R Duldulao;G. Walmsley;Taylor L. Wearda;O. Marecic;M. Longaker;D. Wan

脂肪转移是外科医生治疗全身软组织缺损的重要工具。脂肪是理想的软组织填充物，因为它容易获得，容易获得，便宜，并且具有固有的生物相容性。(1)然而，尽管其迅速普及，脂肪移植是阻碍了不可预测的结果和变量移植存活，与出版的保留率范围从10- 80%。(1-3)为了促进脂肪移植的研究，我们因此开发了一种动物模型，可以实时分析注射的脂肪体积保留。简言之，在CD-1裸小鼠的头皮上切一个小切口，并将200-400 μl处理过的脂肪抽吸物置于颅骨上。选择头皮作为受体部位是因为其缺乏天然的皮下脂肪，并且因为颅骨提供了极好的背景对比度，这有助于分析过程。微型计算机断层扫描（micro-CT）用于在基线和此后每两周扫描移植物。重建CT图像，并使用成像软件来量化移植物体积。传统上，评估脂肪移植物体积的技术需要对研究动物实施安乐死，以通过离体物理测量仅提供移植物重量和体积的单一评估。生化和组织学比较同样要求对研究动物实施安乐死。所描述的成像技术提供了在初始移植后的多个时间点可视化和客观量化体积而不必牺牲研究动物的优点。该技术受到能够注射的移植物尺寸的限制，因为较大的移植物有皮肤和脂肪坏死的风险。该方法适用于所有评估脂肪移植物活力和体积保留的研究。它特别适合于提供脂肪移植物的视觉表示和随时间的体积变化。

Lipotransfer is a vital tool in the surgeon's armamentarium for the treatment of soft tissue deficits of throughout the body. Fat is the ideal soft tissue filler as it is readily available, easily obtained, inexpensive, and inherently biocompatible.(1) However, despite its burgeoning popularity, fat grafting is hampered by unpredictable results and variable graft survival, with published retention rates ranging anywhere from 10-80%. (1-3) To facilitate investigations on fat grafting, we have therefore developed an animal model that allows for real-time analysis of injected fat volume retention. Briefly, a small cut is made in the scalp of a CD-1 nude mouse and 200-400 µl of processed lipoaspirate is placed over the skull. The scalp is chosen as the recipient site because of its absence of native subcutaneous fat, and because of the excellent background contrast provided by the calvarium, which aids in the analysis process. Micro-computed tomography (micro-CT) is used to scan the graft at baseline and every two weeks thereafter. The CT images are reconstructed, and an imaging software is used to quantify graft volumes. Traditionally, techniques to assess fat graft volume have necessitated euthanizing the study animal to provide just a single assessment of graft weight and volume by physical measurement ex vivo. Biochemical and histological comparisons have likewise required the study animal to be euthanized. This described imaging technique offers the advantage of visualizing and objectively quantifying volume at multiple time points after initial grafting without having to sacrifice the study animal. The technique is limited by the size of the graft able to be injected as larger grafts risk skin and fat necrosis. This method has utility for all studies evaluating fat graft viability and volume retention. It is particularly well-suited to providing a visual representation of fat grafts and following changes in volume over time.

Assessment of viability of human fat injection into nude mice with micro-computed tomography.

文献摘要

相似文献