On the regioselectivity of thioether formation by lacticin 481 synthetase
On the regioselectivity of thioether formation by lacticin 481 synthetase
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DOI：
10.1021/ol071301h
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发表时间：
2007-08-16
期刊：
影响因子：
5.2
通讯作者：
van der Donk, Wilfred A.
中科院分区：
文献类型：
--
作者：
Zhang, Xingang;Ni, Weijuan;van der Donk, Wilfred A.
羊毛硫抗生素合成酶通过将半胱氨酸迈克尔型加成到源自 Ser 和 Thr 的脱氢氨基酸上，在肽内产生分子内硫醚交联。这里介绍的是一种可以轻松区分这些交联的酶促和非酶促形成的测定方法。结果明确证明乳酸素 481 合成酶可以产生非天然硫醚交联。
Lantibiotic synthetases generate intramolecular thioether cross-links within peptides through the Michael-type addition of cysteines onto dehydroamino acids originating from Ser and Thr. Presented here is an assay that readily distinguishes between enzymatic and nonenzymatic formation of these cross-links. The results demonstrate unequivocally that lacticin 481 synthetase can generate non-native thioether cross-links.